HBB2/143/17

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$40.00
SKU: HBB2/143/17
View product citations for antibody HBB2/143/17 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: maltase-glucoamylase
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Hauri, H.-P.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Biozentrum, University of Basel, Switzerland
Positive Tested Species Reactivity: Human
Depositors Notes:
Antigen Molecular Weight: Predicted: 210 kDa; Apparent: 335 and 285 kDa (intracellular), 335 kDa (brush border membrane, mature MGA)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MGAM
Immunogen: purified intestinal brush border membrane from normal adult human jejunal mucosa
Alternate Gene Names: MGA, MGAML, MG
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: F0
Epitope Location or Sequence:
Uniprot ID: O43451 
Immunogen Sequence: Full length protein
Entrez Gene ID: 8972 
Additional Characterization:  
Antibody Registry ID: AB_2297616 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HBB2/143/17 was deposited to the DSHB by Hauri, H.-P. (DSHB Hybridoma Product HBB2/143/17)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication
    IF References
    IHC References

    Intestinal brush-border-associated enzymes: co-ordinated expression in colorectal cancer.
    de Bolós C
    International journal of cancer 51.2 (1992 May 8): 173-81.

    IP References

    Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells.
    Marxer A
    The Journal of cell biology 101.3 (1985 Sep): 838-51.

    Human small intestinal maltase-glucoamylase cDNA cloning. Homology to sucrase-isomaltase.
    Sterchi E
    The Journal of biological chemistry 273.5 (1998 Jan 30): 3076-81.

    Structure, biosynthesis, and glycosylation of human small intestinal maltase-glucoamylase.
    Lentze MJ
    The Journal of biological chemistry 263.36 (1988 Dec 25): 19709-17.

    The maltase-glucoamylase gene: common ancestry to sucrase-isomaltase with complementary starch digestion activities.
    Sterchi E
    Proceedings of the National Academy of Sciences of the United States of America 100.3 (2003 Feb 4): 1432-7.

    Human small intestinal angiotensin-converting enzyme: intracellular transport, secretion and glycosylation.
    Naim HY
    The Biochemical journal 296 ( Pt 3). (1993 Dec 15): 607-15.

    ELISA References
    All References

    Intestinal brush-border-associated enzymes: co-ordinated expression in colorectal cancer.
    de Bolós C
    International journal of cancer 51.2 (1992 May 8): 173-81.

    Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells.
    Marxer A
    The Journal of cell biology 101.3 (1985 Sep): 838-51.

    Human small intestinal maltase-glucoamylase cDNA cloning. Homology to sucrase-isomaltase.
    Sterchi E
    The Journal of biological chemistry 273.5 (1998 Jan 30): 3076-81.

    Structure, biosynthesis, and glycosylation of human small intestinal maltase-glucoamylase.
    Lentze MJ
    The Journal of biological chemistry 263.36 (1988 Dec 25): 19709-17.

    The maltase-glucoamylase gene: common ancestry to sucrase-isomaltase with complementary starch digestion activities.
    Sterchi E
    Proceedings of the National Academy of Sciences of the United States of America 100.3 (2003 Feb 4): 1432-7.

    Human small intestinal angiotensin-converting enzyme: intracellular transport, secretion and glycosylation.
    Naim HY
    The Biochemical journal 296 ( Pt 3). (1993 Dec 15): 607-15.

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