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SKU: HBB2/219/20

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DSHB Data Sheet

Catalog Fields

Product Name/ID: HBB2/219/20
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SI
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2188726 
Uniprot ID: P14410 
Entrez Gene ID: 6476 
Clonality: Monoclonal
Immunogen: purified intestinal brush border membrane from normal adult human jejunal mucosa
Immunogen Sequence: Full length protein
Myeloma Strain: F0
Epitope Mapped: No
Antigen Name: sucrase-isomaltase
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/8/2010
Antigen Molecular Weight: Predicted: 209 kDa (pro-SI), 95 kDa (Sucrase), 114 kDa (Isomaltase); Apparent: 231 kDa (pro-SI), 145 kDa (S), 151 kDa (I)
Depositor: Hauri, H.-P.
Antigen Sequence:
Depositor Institution: University of Basel, Biozentrum
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HBB2/219/20 was deposited to the DSHB by Hauri, H.-P. (DSHB Hybridoma Product HBB2/219/20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References

    Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells.
    Marxer A
    The Journal of cell biology 101.3 (1985 Sep): 838-51.

    Dissection of the asynchronous transport of intestinal microvillar hydrolases to the cell surface.
    Hauri HP
    The Journal of cell biology 106.6 (1988 Jun): 1853-61.

    Molecular pathogenicity of novel sucrase-isomaltase mutations found in congenital sucrase-isomaltase deficiency patients.
    Naim HY
    Biochimica et biophysica acta. Molecular basis of disease 1863.3 (2017 Mar): 817-826.

    Lactase and sucrase-isomaltase gene expression during Caco-2 cell differentiation.
    Büller HA
    The Biochemical journal 308 ( Pt 3).Pt 3 (1995 Jun 15): 769-75.

    Immunoblotting with monoclonal antibodies: importance of the blocking solution.
    Bucher K
    Analytical biochemistry 159.2 (1986 Dec): 386-9.

    Human small intestinal angiotensin-converting enzyme: intracellular transport, secretion and glycosylation.
    Naim HY
    The Biochemical journal 296 ( Pt 3).Pt 3 (1993 Dec 15): 607-15.

    ELISA References
    All References

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