HNK-1

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SKU: HNK-1-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: HNK-1
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: CD57, HNK-1, NK-1 Leu-7
Ab Isotype: MIgM, kappa light chain
Gene Name:
Antibody Registry ID: AB_3064988 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Membrane prep T-cell line HSB-2
Clone:
Immunogen Sequence: Native glycoprotein/glycolipid
Myeloma Strain: P3X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: HNK-1 oligosaccharide
Epitope Location or Sequence: sulfated trisaccharide SO4-3GlcA?1-3Gal?1-4GlcNAc
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 110-115 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Human
Depositor Notes: Reacts with human NK and K T-lymphhocyte cells, and tissues of neuroectodermal origin including migrating neural crest. Hybridoma developed by C.M. Balch.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chick, Feline, Human, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HNK-1 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product HNK-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).
    Balch CM
    Journal of immunology (Baltimore, Md. : 1950) 127.3 (1981 Sep): 1024-9.

    IF References

    A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).
    Balch CM
    Journal of immunology (Baltimore, Md. : 1950) 127.3 (1981 Sep): 1024-9.

    WB References

    The distribution and characterization of HNK-1 antigens in the developing avian heart.
    Poelmann RE
    Anatomy and embryology 188.3 (1993 Sep): 307-16.

    IHC References

    HNK-1 antibody detects an antigen expressed on neuroectodermal cells.
    Tursz T
    The Journal of experimental medicine 158.5 (1983 Nov 1): 1775-80.

    The distribution and characterization of HNK-1 antigens in the developing avian heart.
    Poelmann RE
    Anatomy and embryology 188.3 (1993 Sep): 307-16.

    Characterization of HNK-1 antigens during the formation of the avian enteric nervous system.
    Meijers C
    Development (Cambridge, England) 115.2 (1992 Jun): 561-72.

    IP References
    FFPE References

    HNK-1 antibody detects an antigen expressed on neuroectodermal cells.
    Tursz T
    The Journal of experimental medicine 158.5 (1983 Nov 1): 1775-80.

    Characterization of HNK-1 antigens during the formation of the avian enteric nervous system.
    Meijers C
    Development (Cambridge, England) 115.2 (1992 Jun): 561-72.

    FB References

    A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).
    Balch CM
    Journal of immunology (Baltimore, Md. : 1950) 127.3 (1981 Sep): 1024-9.

    Perturbation of cranial neural crest migration by the HNK-1 antibody.
    Bronner-Fraser M
    Developmental biology 123.2 (1987 Oct): 321-31.

    FACS References

    A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).
    Balch CM
    Journal of immunology (Baltimore, Md. : 1950) 127.3 (1981 Sep): 1024-9.

    Epitope Map References
    All References

    HNK-1 antibody detects an antigen expressed on neuroectodermal cells.
    Tursz T
    The Journal of experimental medicine 158.5 (1983 Nov 1): 1775-80.

    The distribution and characterization of HNK-1 antigens in the developing avian heart.
    Poelmann RE
    Anatomy and embryology 188.3 (1993 Sep): 307-16.

    Characterization of HNK-1 antigens during the formation of the avian enteric nervous system.
    Meijers C
    Development (Cambridge, England) 115.2 (1992 Jun): 561-72.

    A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).
    Balch CM
    Journal of immunology (Baltimore, Md. : 1950) 127.3 (1981 Sep): 1024-9.

    Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1.
    Roder JC
    Nature 306.5941 (1983 Nov 24-30): 376-8.

    Perturbation of cranial neural crest migration by the HNK-1 antibody.
    Bronner-Fraser M
    Developmental biology 123.2 (1987 Oct): 321-31.

    Structure of sulfated glucuronyl glycolipids in the nervous system reacting with HNK-1 antibody and some IgM paraproteins in neuropathy.
    Jungalwala FB
    The Journal of biological chemistry 261.25 (1986 Sep 5): 11717-25.

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