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SKU: IFT172.1
View product citations for antibody IFT172.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Intraflagellar transport protein p172
Hybridoma Cells Available: Yes
Antigen Species: Chlamydomonas
Depositor: Rosenbaum, J.L.
Isotype: MIgG1
Depositors Institution: Yale University
Host Species: mouse
Depositors Notes: This antibody is a marker for IFT complex B.
Positive Tested Species Reactivity: Chlamydomonas
Gene: IFT172
Antigen Molecular Weight: apparent 172 kDa; 198 kDa
Clonality: Monoclonal
Immunogen: Purified intraflagellar transport complexes A and B
Epitope Mapped: No
Myeloma Strain: Sp2/0
Additional Information: RRID:AB_2619556
Uniprot ID: Q5DM57 
Entrez Gene ID: 5717250 
Antibody Registry ID: AB_2619556 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IFT172.1 was deposited to the DSHB by Rosenbaum, J.L. (DSHB Hybridoma Product IFT172.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    All References

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