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SKU: IVF12-s
View product citations for antibody IVF12 on CiteAb

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Available: 21

DSHB Data Sheet

Catalog Fields

Antigen: SLC4A1, anion exchanger 1
Hybridoma Cells Available: No
Antigen Species: human
Depositor: Jennings, M.L.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Arkansas for Medical Sciences
Positive Tested Species Reactivity: Chicken, Mammal, Rat
Depositors Notes:
Antigen Molecular Weight: 52 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SLC4A1
Immunogen: Anion Exchanger1 (Band 3)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence: epitope is cytoplasmic
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2191318 
Additional Information: RRID:AB_2191318
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IVF12 was deposited to the DSHB by Jennings, M.L. (DSHB Hybridoma Product IVF12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References

    Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1.
    Mitchell BJ
    The Journal of cell biology 206.3 (2014 Aug 4): 367-76.

    Two types of collecting duct mitochondria-rich (intercalated) cells: lectin and band 3 cytochemistry.
    Jennings ML
    The American journal of physiology 251.3 Pt 1 (1986 Sep): C347-55.

    IHC References

    MiRP3 acts as an accessory subunit with the BK potassium channel.
    Goldstein SA
    American journal of physiology. Renal physiology 295.2 (2008 Aug): F380-7.

    All References

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