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SKU: IVF12-s
View product citations for antibody IVF12 on CiteAb

In Stock

Available: 11

DSHB Data Sheet

Catalog Fields

Antigen: SLC4A1, anion exchanger 1
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG2a
Depositor: Jennings, M.L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chicken, Mammal, Rat
Depositors Institution: University of Arkansas for Medical Sciences
Antigen Molecular Weight: 52 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Anion Exchanger1 (Band 3)
Gene: SLC4A1
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence: epitope is cytoplasmic
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_2191318 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: RRID:AB_2191318
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
IVF12 was deposited to the DSHB by Jennings, M.L. (DSHB Hybridoma Product IVF12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References

    Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1.
    Mitchell BJ
    The Journal of cell biology 206.3 (2014 Aug 4): 367-76.

    Two types of collecting duct mitochondria-rich (intercalated) cells: lectin and band 3 cytochemistry.
    Jennings ML
    The American journal of physiology 251.3 Pt 1 (1986 Sep): C347-55.

    IHC References

    MiRP3 acts as an accessory subunit with the BK potassium channel.
    Goldstein SA
    American journal of physiology. Renal physiology 295.2 (2008 Aug): F380-7.

    All References

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