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$45.00 to $425.00
SKU: JG1.1.20
View product citations for antibody JG1.1.20 on CiteAb

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Available: 5

DSHB Data Sheet

Catalog Fields

Antigen: addressin (PNAd)
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Butcher, E.C.
Isotype: RIgG2c
Antigen Sequence:
Host Species: rat
Depositors Institution: Stanford University Medical Center / VA Palo Alto Health Care Systems
Positive Tested Species Reactivity: Human
Depositors Notes: Does not block L-selectin binding of human lymphocytes. positive on human tonsil HEV and P.L.N. HEV. negative on human appendix HEV and chronically inflamed skin. can cross-block with other JG Abs on tonsil sections; can cross-block with other JG Abs and MECA-79 on purified PNAd in ELISAS. does not recognize mouse PNAd, only human PNAd. neuraminidase sensitive, sialic acid-dependent. immunoisolated by monoclonal Ab affinity chromatography; SDS page gel, Western blot. polyacrylamide gel electrophoresis to nitrocellulose membrane via Trans blot; alkaline phosphatase IgG, then Western blot substrate. tissue extracts passed over agarose, then monoclonal Ab affinity column. purity assessed by iodination followed by SDS page and autoradiography. rolling assays-done only with materials isolated from lmphoid tissues, not from in-vitro cultured cells. frozen sections of human tonsils, p.l.n., appendix, or inflamed skin incubated with JG supernatant, PE anti-rat IgG, and FITC conjugated MECA-79.
Antigen Molecular Weight: mostly about 150 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: human PNAd
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528435 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JG1.1.20 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product JG1.1.20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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