JG1.1.20
Catalog Fields
Clone ID/Product Name: JG1.1.20
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: RIgG2c
Gene Name:
Antibody Registry ID: AB_528435
Clonality: Monoclonal
Immunogen: human PNAd
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: addressin (PNAd)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/8/1998
Antigen Molecular Weight: mostly about 150 kDa
Depositor: Butcher, E.C.
Antigen Sequence:
Depositor Institution: Stanford University Medical Center / VA Palo Alto Health Care Systems
Antigen Species: human
Depositor Notes: Does not block L-selectin binding of human lymphocytes. positive on human tonsil HEV and P.L.N. HEV. negative on human appendix HEV and chronically inflamed skin. can cross-block with other JG Abs on tonsil sections; can cross-block with other JG Abs and MECA-79 on purified PNAd in ELISAS. does not recognize mouse PNAd, only human PNAd. neuraminidase sensitive, sialic acid-dependent. immunoisolated by monoclonal Ab affinity chromatography; SDS page gel, Western blot. polyacrylamide gel electrophoresis to nitrocellulose membrane via Trans blot; alkaline phosphatase IgG, then Western blot substrate. tissue extracts passed over agarose, then monoclonal Ab affinity column. purity assessed by iodination followed by SDS page and autoradiography. rolling assays-done only with materials isolated from lmphoid tissues, not from in-vitro cultured cells. frozen sections of human tonsils, p.l.n., appendix, or inflamed skin incubated with JG supernatant, PE anti-rat IgG, and FITC conjugated MECA-79.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JG1.1.20 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product JG1.1.20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Complexity and differential expression of carbohydrate epitopes associated with L-selectin recognition of high endothelial venules.
Butcher EC
The American journal of pathology 152.2 (1998 Feb): 469-77.