Clone ID / Name: JG1.1.20

Anti - addressin (PNAd)

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$50.00

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SKU: JG1.1.20

Product Form :

Supernatant 1.0 ml

~ 10 – 38 μg/mL

Frozen Cells 1 vial

N/A

Growing Cells 1 vial

N/A

iConcentration varies between lots. Lots are distributed in the order they are produced.

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Available: 1

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Catalog Fields

Identity

Clone ID / Name: JG1.1.20

Alternate Antibody Name: —

Antigen Name: addressin (PNAd)

Alternate Antigen Name: —

Gene Symbol: —

Gene Name: —

Entrez Gene ID: —

Antigen Sequence: —

Antigen Species: human

Antigen Mol. Weight: mostly about 150 kDa

Uniprot ID: —

Human Protein Atlas: —

Antibody Properties

Host Species: Rat

Clonality: Monoclonal

Isotype: Rat IgG2c

Myeloma Strain: SP2/0

Antibody Registry / RRID: AB_528435

Available to For-Profits: Yes

Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.

Immunogen & Epitope

Immunogen: human PNAd

Immunogen Sequence: —

Epitope Mapped: No

Epitope Location / Seq: —

Clone: —

Additional Info: —

Add. Characterization: —

Species Reactivity

Confirmed Reactivity: Human

Predicted Reactivity: —

Depositor

Depositor Name: E.C. Butcher

Institution: Stanford University Medical Center / VA Palo Alto Health Care Systems

Deposit Date: June 8, 1998

Depositor Notes: Does not block L-selectin binding of human lymphocytes. positive on human tonsil HEV and P.L.N. HEV. negative on human appendix HEV and chronically inflamed skin. can cross-block with other JG Abs on tonsil sections; can cross-block with other JG Abs and MECA-79 on purified PNAd in ELISAS. does not recognize mouse PNAd, only human PNAd. neuraminidase sensitive, sialic acid-dependent. immunoisolated by monoclonal Ab affinity chromatography; SDS page gel, Western blot. polyacrylamide gel electrophoresis to nitrocellulose membrane via Trans blot; alkaline phosphatase IgG, then Western blot substrate. tissue extracts passed over agarose, then monoclonal Ab affinity column. purity assessed by iodination followed by SDS page and autoradiography. rolling assays-done only with materials isolated from lmphoid tissues, not from in-vitro cultured cells. frozen sections of human tonsils, p.l.n., appendix, or inflamed skin incubated with JG supernatant, PE anti-rat IgG, and FITC conjugated MECA-79.

Recommended Applications

IHC

Each lab must optimize concentration per application and species. Full usage recommendations →

UniProt DataUniProtNo UniProt ID

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No UniProt ID is available for this product.

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Clone ID / Name: JG1.1.20

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