JG22

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$40.00
SKU: JG22
View product citations for antibody JG22 on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: laminin/fibronectin receptor
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Gottlieb, D.I.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University School of Medicine
Positive Tested Species Reactivity: Chick
Depositors Notes: Cell binding note: many cell types of chick, including muscle and fibroblasts. Functional effects: perturbs cell-substrate attachment.
Antigen Molecular Weight: about 140 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: chick muscle tissue
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications:  
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JG22 was deposited to the DSHB by Gottlieb, D.I. (DSHB Hybridoma Product JG22)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibodies which alter the morphology of cultured chick myogenic cells.
    Gottlieb DI
    Journal of cellular biochemistry 18.2 (1982): 221-9.

    All References

    Monoclonal antibodies which alter the morphology of cultured chick myogenic cells.
    Gottlieb DI
    Journal of cellular biochemistry 18.2 (1982): 221-9.

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