JONES

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$40.00
SKU: JONES-s
View product citations for antibody JONES on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neural retinal gangliosides (9-0-acetyl-GD3)
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Constantine-Paton, M.
Isotype: MIgM
Depositors Institution: Massachusetts Institute of Technology
Host Species: mouse
Depositors Notes: JONES doesn't react with chicken tissue. It recognizes 9-0-acetyl-GD3 and a base-labile derivative of GQ1C, present in chloroform/methanol extracts from perinatal retina; neuraminidase sensitive. By immunohistochemistry, JONES binds to aldehyde-fixed sections of late fetal and neonatal rat retina.
Positive Tested Species Reactivity: Cat, Goat, Human, Monkey, Mouse, Opossum, Rat
Clonality: Monoclonal
Immunogen: Retinal epithelia homogenate (lightly fixed: 2% paraformaldehyde for 30 min) of fetal rats (E13-E20)
Epitope Mapped: Yes
Myeloma Strain: P3-NS1/1-Ag4-1
Epitope Location or Sequence: 9-0-acetyl-GD3
Antibody Registry ID: AB_531870 
Immunogen Sequence: GD3
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JONES was deposited to the DSHB by Constantine-Paton, M. (DSHB Hybridoma Product JONES)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina.
    Barnstable CJ
    Nature 324.6096 (1986 Dec 4-10): 459-62.

    A developmentally regulated antigen associated with neural cell and process migration.
    Constantine-Paton M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 564-79.

    Developmental regulation of ganglioside antigens recognized by the JONES antibody.
    Constantine-Paton M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 580-92.

    Granule cell induction of 9-O-acetyl gangliosides on cerebellar glia in microcultures.
    Constantine-Paton M
    Developmental biology 138.2 (1990 Apr): 400-9.

    Differential recognition of a set of O-acetylated gangliosides by monoclonal antibodies RB13-2, D1.1, and JONES during rat brain development.
    Rajewsky MF
    Brain research. Developmental brain research 51.2 (1990 Feb 1): 279-82.

    A gradient molecule in developing rat retina: expression of 9-O-acetyl GD3 in relation to cell type, developmental age, and GD3 ganglioside.
    Barnstable CJ
    Journal of neuroscience research 21.2-4 (1988 Oct-Dec): 398-409.

    O-acetylation of a cell-surface carbohydrate creates discrete molecular patterns during neural development.
    Barnstable CJ
    Proceedings of the National Academy of Sciences of the United States of America 84.23 (1987 Dec): 8716-20.

    JONES ganglioside expression on retinal glia increases after axotomy.
    Schlosshauer B
    Journal of neurocytology 18.4 (1989 Aug): 553-63.

    Expression and function of ganglioside 9-O-acetyl GD3 in postmitotic granule cell development.
    Mendez-Otero R
    Molecular and cellular neurosciences 17.3 (2001 Mar): 488-99.

    Expression of 9-O-acetylated gangliosides during development of the rat olfactory system.
    Ramon-Cueto A
    Neuroreport 5.14 (1994 Sep 8): 1755-9.

    WB References

    A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina.
    Barnstable CJ
    Nature 324.6096 (1986 Dec 4-10): 459-62.

    Structural and immunological characterization of O-acetylated GD2. Evidence that GD2 is an acceptor for ganglioside O-acetyltransferase in human melanoma cells.
    Varki A
    The Journal of biological chemistry 267.23 (1992 Aug 15): 16200-11.

    Developmental regulation of ganglioside antigens recognized by the JONES antibody.
    Constantine-Paton M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 580-92.

    Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk.
    Yu RK
    Lipids 24.8 (1989 Aug): 680-4.

    Differential recognition of a set of O-acetylated gangliosides by monoclonal antibodies RB13-2, D1.1, and JONES during rat brain development.
    Rajewsky MF
    Brain research. Developmental brain research 51.2 (1990 Feb 1): 279-82.

    O-acetylation of a cell-surface carbohydrate creates discrete molecular patterns during neural development.
    Barnstable CJ
    Proceedings of the National Academy of Sciences of the United States of America 84.23 (1987 Dec): 8716-20.

    IHC References
    FB References

    Expression and function of ganglioside 9-O-acetyl GD3 in postmitotic granule cell development.
    Mendez-Otero R
    Molecular and cellular neurosciences 17.3 (2001 Mar): 488-99.

    Epitope Map References

    O-acetylation of a cell-surface carbohydrate creates discrete molecular patterns during neural development.
    Barnstable CJ
    Proceedings of the National Academy of Sciences of the United States of America 84.23 (1987 Dec): 8716-20.

    Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk.
    Yu RK
    Lipids 24.8 (1989 Aug): 680-4.

    All References

    A gradient molecule in developing rat retina: expression of 9-O-acetyl GD3 in relation to cell type, developmental age, and GD3 ganglioside.
    Barnstable CJ
    Journal of neuroscience research 21.2-4 (1988 Oct-Dec): 398-409.

    Expression of 9-O-acetylated gangliosides is correlated with tangential cell migration in the rat brain.
    Cavalcante LA
    Neuroscience letters 204.1-2 (1996 Feb 2): 97-100.

    Expression of 9-O-acetylated gangliosides during development of the rat olfactory system.
    Ramon-Cueto A
    Neuroreport 5.14 (1994 Sep 8): 1755-9.

    A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina.
    Barnstable CJ
    Nature 324.6096 (1986 Dec 4-10): 459-62.

    A developmentally regulated antigen associated with neural cell and process migration.
    Constantine-Paton M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 564-79.

    Developmental regulation of ganglioside antigens recognized by the JONES antibody.
    Constantine-Paton M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 580-92.

    Granule cell induction of 9-O-acetyl gangliosides on cerebellar glia in microcultures.
    Constantine-Paton M
    Developmental biology 138.2 (1990 Apr): 400-9.

    Differential recognition of a set of O-acetylated gangliosides by monoclonal antibodies RB13-2, D1.1, and JONES during rat brain development.
    Rajewsky MF
    Brain research. Developmental brain research 51.2 (1990 Feb 1): 279-82.

    O-acetylation of a cell-surface carbohydrate creates discrete molecular patterns during neural development.
    Barnstable CJ
    Proceedings of the National Academy of Sciences of the United States of America 84.23 (1987 Dec): 8716-20.

    JONES ganglioside expression on retinal glia increases after axotomy.
    Schlosshauer B
    Journal of neurocytology 18.4 (1989 Aug): 553-63.

    Expression and function of ganglioside 9-O-acetyl GD3 in postmitotic granule cell development.
    Mendez-Otero R
    Molecular and cellular neurosciences 17.3 (2001 Mar): 488-99.

    Structural and immunological characterization of O-acetylated GD2. Evidence that GD2 is an acceptor for ganglioside O-acetyltransferase in human melanoma cells.
    Varki A
    The Journal of biological chemistry 267.23 (1992 Aug 15): 16200-11.

    Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk.
    Yu RK
    Lipids 24.8 (1989 Aug): 680-4.

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