K14/16

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$40.00
SKU: K14/16
View product citations for antibody K14/16 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Kv1.2 K+ channel/Potassium voltage-gated channel subfamily A member 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Trimmer, J.S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York, Stony Brook
Positive Tested Species Reactivity: Human, Mouse, Rat, Xenopus, Zebrafish
Depositors Notes: K14/16 does not cross-react with other Kv1 channels.
Antigen Molecular Weight: 80 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: KCNA2
Immunogen: Fusion protein, cytoplasmic C-terminus
Alternate Gene Names: EIEE32, HBK5, HK4, HUKIV, KV1.2, MK2, NGK1, RBK2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: beta-subunit a.a. 463-480
Uniprot ID: P16389 
Immunogen Sequence: a.a. 428-499
Entrez Gene ID: 3737 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K14/16 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K14/16)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ChIP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides.
    Trimmer JS
    Neuropharmacology 35.7 (1996): 851-65.

    Beta subunits promote K+ channel surface expression through effects early in biosynthesis.
    Trimmer JS
    Neuron 16.4 (1996 Apr): 843-52.

    Potassium channel distribution, clustering, and function in remyelinating rat axons.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.1 (1998 Jan 1): 36-47.

    Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.
    Trimmer JS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.21 (1997 Nov 1): 8246-58.

    Dynamic potassium channel distributions during axonal development prevent aberrant firing patterns.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.2 (1999 Jan 15): 747-58.

    Subunit composition determines Kv1 potassium channel surface expression.
    Trimmer JS
    The Journal of biological chemistry 275.38 (2000 Sep 22): 29685-93.

    Kv beta subunit oxidoreductase activity and Kv1 potassium channel trafficking.
    Trimmer JS
    The Journal of biological chemistry 277.10 (2002 Mar 8): 8298-305.

    Light and electron microscopic analysis of KChIP and Kv4 localization in rat cerebellar granule cells.
    Trimmer JS
    The Journal of comparative neurology 484.2 (2005 Apr 4): 144-55.

    Localization of the KCC4 potassium-chloride cotransporter in the nervous system.
    Delpire E
    Neuroscience 123.2 (2004): 381-91.

    WB References

    Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides.
    Trimmer JS
    Neuropharmacology 35.7 (1996): 851-65.

    Beta subunits promote K+ channel surface expression through effects early in biosynthesis.
    Trimmer JS
    Neuron 16.4 (1996 Apr): 843-52.

    Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.
    Trimmer JS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.21 (1997 Nov 1): 8246-58.

    Subunit composition determines Kv1 potassium channel surface expression.
    Trimmer JS
    The Journal of biological chemistry 275.38 (2000 Sep 22): 29685-93.

    Kv beta subunit oxidoreductase activity and Kv1 potassium channel trafficking.
    Trimmer JS
    The Journal of biological chemistry 277.10 (2002 Mar 8): 8298-305.

    IHC References

    Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.
    Trimmer JS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.21 (1997 Nov 1): 8246-58.

    Light and electron microscopic analysis of KChIP and Kv4 localization in rat cerebellar granule cells.
    Trimmer JS
    The Journal of comparative neurology 484.2 (2005 Apr 4): 144-55.

    ChIP References

    Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.
    Trimmer JS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.21 (1997 Nov 1): 8246-58.

    Nogo-A at CNS paranodes is a ligand of Caspr: possible regulation of K(+) channel localization.
    Xiao ZC
    The EMBO journal 22.21 (2003 Nov 3): 5666-78.

    ELISA References
    Epitope Map References
    All References

    Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.
    Trimmer JS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 17.21 (1997 Nov 1): 8246-58.

    Light and electron microscopic analysis of KChIP and Kv4 localization in rat cerebellar granule cells.
    Trimmer JS
    The Journal of comparative neurology 484.2 (2005 Apr 4): 144-55.

    Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides.
    Trimmer JS
    Neuropharmacology 35.7 (1996): 851-65.

    Beta subunits promote K+ channel surface expression through effects early in biosynthesis.
    Trimmer JS
    Neuron 16.4 (1996 Apr): 843-52.

    Potassium channel distribution, clustering, and function in remyelinating rat axons.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.1 (1998 Jan 1): 36-47.

    Dynamic potassium channel distributions during axonal development prevent aberrant firing patterns.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.2 (1999 Jan 15): 747-58.

    Subunit composition determines Kv1 potassium channel surface expression.
    Trimmer JS
    The Journal of biological chemistry 275.38 (2000 Sep 22): 29685-93.

    Kv beta subunit oxidoreductase activity and Kv1 potassium channel trafficking.
    Trimmer JS
    The Journal of biological chemistry 277.10 (2002 Mar 8): 8298-305.

    Localization of the KCC4 potassium-chloride cotransporter in the nervous system.
    Delpire E
    Neuroscience 123.2 (2004): 381-91.

    Nogo-A at CNS paranodes is a ligand of Caspr: possible regulation of K(+) channel localization.
    Xiao ZC
    The EMBO journal 22.21 (2003 Nov 3): 5666-78.

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