K20/78

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$45.00
SKU: K20/78
View product citations for antibody K20/78 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Kv1.1 K+ channel/ Potassium voltage-gated channel subfamily A member 1 /
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York, Stony Brook
Positive Tested Species Reactivity: C. elegans, Human, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: 85 kDa (mature); 65 kDa (immature)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Kcna1
Immunogen: Cytoplasmic C-terminus synthetic peptide
Alternate Gene Names: Kcna, Kcpvd, Kv1.1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: alpha subunit cytoplasmic domain
Uniprot ID: P10499 
Immunogen Sequence: a.a. 458-476
Entrez Gene ID: 24520 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K20/78 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K20/78)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides.
    Trimmer JS
    Neuropharmacology 35.7 (1996): 851-65.

    PSD-95 and SAP97 exhibit distinct mechanisms for regulating K(+) channel surface expression and clustering.
    Trimmer JS
    The Journal of cell biology 148.1 (2000 Jan 10): 147-58.

    Potassium channel distribution, clustering, and function in remyelinating rat axons.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.1 (1998 Jan 1): 36-47.

    Subunit composition determines Kv1 potassium channel surface expression.
    Trimmer JS
    The Journal of biological chemistry 275.38 (2000 Sep 22): 29685-93.

    Dynamic potassium channel distributions during axonal development prevent aberrant firing patterns.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.2 (1999 Jan 15): 747-58.

    Unique role of dystroglycan in peripheral nerve myelination, nodal structure, and sodium channel stabilization.
    Campbell KP
    Neuron 38.5 (2003 Jun 5): 747-58.

    Syndecan-3 and syndecan-4 are enriched in Schwann cell perinodal processes.
    Girault JA
    BMC neuroscience 4. (2003 Nov 18): 29.

    WB References
    IHC References
    IP References
    ELISA References
    Epitope Map References
    All References

    Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides.
    Trimmer JS
    Neuropharmacology 35.7 (1996): 851-65.

    PSD-95 and SAP97 exhibit distinct mechanisms for regulating K(+) channel surface expression and clustering.
    Trimmer JS
    The Journal of cell biology 148.1 (2000 Jan 10): 147-58.

    Potassium channel distribution, clustering, and function in remyelinating rat axons.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.1 (1998 Jan 1): 36-47.

    Subunit composition determines Kv1 potassium channel surface expression.
    Trimmer JS
    The Journal of biological chemistry 275.38 (2000 Sep 22): 29685-93.

    Dynamic potassium channel distributions during axonal development prevent aberrant firing patterns.
    Shrager P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.2 (1999 Jan 15): 747-58.

    Unique role of dystroglycan in peripheral nerve myelination, nodal structure, and sodium channel stabilization.
    Campbell KP
    Neuron 38.5 (2003 Jun 5): 747-58.

    Syndecan-3 and syndecan-4 are enriched in Schwann cell perinodal processes.
    Girault JA
    BMC neuroscience 4. (2003 Nov 18): 29.

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