K28/43

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$40.00
SKU: K28/43
View product citations for antibody K28/43 on CiteAb

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Available: 26

DSHB Data Sheet

Catalog Fields

Antigen: PSD-95 MAGUK scaffold protein
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Trimmer, J.S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York, Stony Brook
Positive Tested Species Reactivity: African green monkey, Human, Mouse, Rabbit, Rat
Depositors Notes: Does not cross-react with DLG1/SAP97, DLG2/Chapsyn-110 or DLG3/SAP102
Antigen Molecular Weight: 95-110 kDa (variable due to phosphorylation)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: DLG4
Immunogen: Fusion protein of PDZ domains 1 and 2
Alternate Gene Names: PSD95, SAP-90, SAP90
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P78352 
Immunogen Sequence: a.a. 77-299
Entrez Gene ID: 1742 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K28/43 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K28/43)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Developmental clustering of ion channels at and near the node of Ranvier.
    Trimmer JS
    Developmental biology 236.1 (2001 Aug 1): 5-16.

    IF References
    WB References

    Clustering of neuronal potassium channels is independent of their interaction with PSD-95.
    Trimmer JS
    The Journal of cell biology 159.4 (2002 Nov 25): 663-72.

    A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
    Trimmer JS
    The Journal of biological chemistry 278.38 (2003 Sep 19): 36445-54.

    Lipid rafts in the maintenance of synapses, dendritic spines, and surface AMPA receptor stability.
    Sheng M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.8 (2003 Apr 15): 3262-71.

    Differential roles of NR2A- and NR2B-containing NMDA receptors in Ras-ERK signaling and AMPA receptor trafficking.
    Sheng M
    Neuron 46.5 (2005 Jun 2): 745-60.

    Expression, regulation and role of the MAGUK protein SAP-97 in human atrial myocardium.
    Hatem SN
    Cardiovascular research 56.3 (2002 Dec): 433-42.

    IP References

    Clustering of neuronal potassium channels is independent of their interaction with PSD-95.
    Trimmer JS
    The Journal of cell biology 159.4 (2002 Nov 25): 663-72.

    A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
    Trimmer JS
    The Journal of biological chemistry 278.38 (2003 Sep 19): 36445-54.

    Expression, regulation and role of the MAGUK protein SAP-97 in human atrial myocardium.
    Hatem SN
    Cardiovascular research 56.3 (2002 Dec): 433-42.

    All References

    Clustering of neuronal potassium channels is independent of their interaction with PSD-95.
    Trimmer JS
    The Journal of cell biology 159.4 (2002 Nov 25): 663-72.

    A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
    Trimmer JS
    The Journal of biological chemistry 278.38 (2003 Sep 19): 36445-54.

    Activity-dependent redistribution and essential role of cortactin in dendritic spine morphogenesis.
    Sheng M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.37 (2003 Dec 17): 11759-69.

    Differential roles of NR2A- and NR2B-containing NMDA receptors in Ras-ERK signaling and AMPA receptor trafficking.
    Sheng M
    Neuron 46.5 (2005 Jun 2): 745-60.

    Quaternary structure, protein dynamics, and synaptic function of SAP97 controlled by L27 domain interactions.
    Sheng M
    Neuron 44.3 (2004 Oct 28): 453-67.

    Lipid rafts in the maintenance of synapses, dendritic spines, and surface AMPA receptor stability.
    Sheng M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.8 (2003 Apr 15): 3262-71.

    Expression, regulation and role of the MAGUK protein SAP-97 in human atrial myocardium.
    Hatem SN
    Cardiovascular research 56.3 (2002 Dec): 433-42.

    Developmental clustering of ion channels at and near the node of Ranvier.
    Trimmer JS
    Developmental biology 236.1 (2001 Aug 1): 5-16.

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