K28/86

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$45.00 to $425.00
SKU: K28/86
View product citations for antibody K28/86 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Pan-MAGUK scaffold proteins
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Trimmer, J.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. elegans, Human, Mouse, Rat, Xenopus, Zebrafish
Depositors Institution: UC - Davis
Antigen Molecular Weight: 95-110 kDa
Depositors Notes: This antibody cross-reacts with DLG1/SAP97, DLG2/Chapsyn-110 and DLG3/SAP102. Molecular weight varies due to phosphorylation.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: GST-fusion protein (PDZ domains 1 & 2)
Gene: DLG4
Alternate Antibody Name:
Alternate Gene Names: MRD62, PSD95, SAP-90, SAP90
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: P78352 
Epitope Location or Sequence:
Entrez Gene ID: 1742 
Immunogen Sequence: a.a. 77-299
Antibody Registry ID:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K28/86 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K28/86)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Capabilities of neurexins in the chick ciliary ganglion.
    Conroy WG
    Developmental neurobiology 68.3 (2008 Feb 15): 409-19.

    IF References

    Capabilities of neurexins in the chick ciliary ganglion.
    Conroy WG
    Developmental neurobiology 68.3 (2008 Feb 15): 409-19.

    IHC References
    All References

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