K28/86

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$40.00
SKU: K28/86
View product citations for antibody K28/86 on CiteAb

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Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: Pan-MAGUK scaffold proteins
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: C. elegans, Human, Mouse, Rat, Xenopus, Zebrafish
Depositors Notes: This antibody cross-reacts with DLG1/SAP97, DLG2/Chapsyn-110 and DLG3/SAP102. Molecular weight varies due to phosphorylation.
Antigen Molecular Weight: 95-110 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: DLG4
Immunogen: GST-fusion protein (PDZ domains 1 & 2)
Alternate Gene Names: MRD62, PSD95, SAP-90, SAP90
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P78352 
Immunogen Sequence: a.a. 77-299
Entrez Gene ID: 1742 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K28/86 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K28/86)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Capabilities of neurexins in the chick ciliary ganglion.
    Conroy WG
    Developmental neurobiology 68.3 (2008 Feb 15): 409-19.

    IF References

    Capabilities of neurexins in the chick ciliary ganglion.
    Conroy WG
    Developmental neurobiology 68.3 (2008 Feb 15): 409-19.

    IHC References
    All References

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