K57/1

(0) No Reviews yet
$45.00
SKU: K57/1
View product citations for antibody K57/1 on CiteAb

In Stock

Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: Kv4.2 K+ channel (external)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York, Stony Brook
Positive Tested Species Reactivity: C. elegans, Mouse, Rat, Xenopus
Depositors Notes: Does not cross-react with Kv4.3
Antigen Molecular Weight: 70 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Kcnd2
Immunogen: Extracellular S1-S2 loop synthetic peptide
Alternate Gene Names: Kv4.2, RK5, Shal1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: ectodomain of alpha subunit
Uniprot ID: Q9NZV8 
Immunogen Sequence: a.a. 209-225
Entrez Gene ID: 65180 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K57/1 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K57/1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
    Trimmer JS
    The Journal of biological chemistry 278.38 (2003 Sep 19): 36445-54.

    IF References
    WB References

    A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
    Trimmer JS
    The Journal of biological chemistry 278.38 (2003 Sep 19): 36445-54.

    Calcium- and metabolic state-dependent modulation of the voltage-dependent Kv2.1 channel regulates neuronal excitability in response to ischemia.
    Trimmer JS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 25.48 (2005 Nov 30): 11184-93.

    IP References

    A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
    Trimmer JS
    The Journal of biological chemistry 278.38 (2003 Sep 19): 36445-54.

    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review