K65/35

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$40.00
SKU: K65/35
View product citations for antibody K65/35 on CiteAb

In Stock

Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: Contactin-associated protein 1/ CASPR/Neurexin IV
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Trimmer, J.S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Caspr KO sample courtesy of Drs Ido Horresh and Ori Peles, The Weizmann Institute of Science.
Antigen Molecular Weight: 220 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Cntnap1
Immunogen: Recombinant rat cytoplasmic domain GST fusion protein
Alternate Gene Names: CASPR, CHN3, CNTNAP, NRXN4, P190
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P97846 
Immunogen Sequence: a.a.1308-1381 (cytoplasmic)
Entrez Gene ID: 84008 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K65/35 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K65/35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • All References
  • Initial Publication

    Subunit composition and novel localization of K+ channels in spinal cord.
    Trimmer JS
    The Journal of comparative neurology 429.1 (2001 Jan 1): 166-76.

    IF References
    WB References

    Molecular microdomains in a sensory terminal, the vestibular calyx ending.
    Eatock RA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.27 (2011 Jul 6): 10101-14.

    Molecular microdomains in a sensory terminal, the vestibular calyx ending.
    Eatock RA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.27 (2011 Jul 6): 10101-14.

    In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons.
    Bordey A
    Neuroscience research 143. (2019 Jun): 44-52.

    IHC References

    Secretome Analysis of Mesenchymal Stem Cell Factors Fostering Oligodendroglial Differentiation of Neural Stem Cells In Vivo.
    Küry P
    International journal of molecular sciences 21.12 (2020 Jun 18): .

    Ankyrin-G regulates forebrain connectivity and network synchronization via interaction with GABARAP.
    Jenkins PM
    Molecular psychiatry 25.11 (2020 Nov): 2800-2817.

    Spatiotemporal development of spinal neuronal and glial populations in the Ts65Dn mouse model of Down syndrome.
    Haydar TF
    Journal of neurodevelopmental disorders 11.1 (2019 Dec 16): 35.

    Experimental Traumatic Brain Injury Identifies Distinct Early and Late Phase Axonal Conduction Deficits of White Matter Pathophysiology, and Reveals Intervening Recovery.
    Armstrong RC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 38.41 (2018 Oct 10): 8723-8736.

    Molecular microdomains in a sensory terminal, the vestibular calyx ending.
    Eatock RA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.27 (2011 Jul 6): 10101-14.

    Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily.
    Waxman SG
    The European journal of neuroscience 23.10 (2006 May): 2551-62.

    Purification and Characterization of Schwann Cells from Adult Human Skin and Nerve.
    Biernaskie J
    eNeuro 4.3 (2017 May-Jun): .

    Molecular microdomains in a sensory terminal, the vestibular calyx ending.
    Eatock RA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.27 (2011 Jul 6): 10101-14.

    IP References

    Subunit composition and novel localization of K+ channels in spinal cord.
    Trimmer JS
    The Journal of comparative neurology 429.1 (2001 Jan 1): 166-76.

    ELISA References

    Subunit composition and novel localization of K+ channels in spinal cord.
    Trimmer JS
    The Journal of comparative neurology 429.1 (2001 Jan 1): 166-76.

    FFPE References
    All References

    Secretome Analysis of Mesenchymal Stem Cell Factors Fostering Oligodendroglial Differentiation of Neural Stem Cells In Vivo.
    Küry P
    International journal of molecular sciences 21.12 (2020 Jun 18): .

    Ankyrin-G regulates forebrain connectivity and network synchronization via interaction with GABARAP.
    Jenkins PM
    Molecular psychiatry 25.11 (2020 Nov): 2800-2817.

    Spatiotemporal development of spinal neuronal and glial populations in the Ts65Dn mouse model of Down syndrome.
    Haydar TF
    Journal of neurodevelopmental disorders 11.1 (2019 Dec 16): 35.

    Experimental Traumatic Brain Injury Identifies Distinct Early and Late Phase Axonal Conduction Deficits of White Matter Pathophysiology, and Reveals Intervening Recovery.
    Armstrong RC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 38.41 (2018 Oct 10): 8723-8736.

    Molecular microdomains in a sensory terminal, the vestibular calyx ending.
    Eatock RA
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.27 (2011 Jul 6): 10101-14.

    Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily.
    Waxman SG
    The European journal of neuroscience 23.10 (2006 May): 2551-62.

    Purification and Characterization of Schwann Cells from Adult Human Skin and Nerve.
    Biernaskie J
    eNeuro 4.3 (2017 May-Jun): .

    Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model.
    Alshammari TK
    Neural plasticity 2019. (2019): 4893103.

    mTORC1 Activation by Loss of <i>Tsc1</i> in Myelinating Glia Causes Downregulation of Quaking and Neurofascin 155 Leading to Paranodal Domain Disorganization.
    Bhat MA
    Frontiers in cellular neuroscience 12. (2018): 201.

    A histone deacetylase 3-dependent pathway delimits peripheral myelin growth and functional regeneration.
    Lu QR
    Nature medicine 24.3 (2018 Mar): 338-351.

    In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons.
    Bordey A
    Neuroscience research 143. (2019 Jun): 44-52.

    Subunit composition and novel localization of K+ channels in spinal cord.
    Trimmer JS
    The Journal of comparative neurology 429.1 (2001 Jan 1): 166-76.

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