K75/41
Catalog Fields
Clone ID/Product Name: K75/41
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: KCND3
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2314723
Uniprot ID: Q62897
Entrez Gene ID: 3752
Clonality: Monoclonal
Immunogen: Recombinant fusion protein
Clone:
Immunogen Sequence: a.a. 415-636 (C-terminus)
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Kv4.3 K+ channel
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/21/2019
Antigen Molecular Weight: 70 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Rat
Depositor Notes: This antibody does not cross-react with Kv4.2.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat, Xenopus
Additional Information: K75/41 specificity has been validated in KCND3 KO mice. PMID: 18045613
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K75/41 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K75/41)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
9 References
Initial Publication
KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain.
Trimmer JS
The Journal of neuroscience : the official journal of the Society for Neuroscience 24.36 (2004 Sep 8): 7903-15.