K89/34

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$40.00
SKU: K89/34
View product citations for antibody K89/34 on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: Kv2.1 K+ channel
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York, Stony Brook
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: No cross-reactivity against rat Kv2.2
Antigen Molecular Weight: 105-125 kDa (variable due to phosphorylation)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: KCNB1
Immunogen: Cytoplasmic C-terminus synthetic peptide
Alternate Gene Names: Shab; Kv2.1; DRK1PC; Kcr1-1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P15387 
Immunogen Sequence: a.a. 837-853
Entrez Gene ID: 25736 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
K89/34 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product K89/34)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    A primary culture system for biochemical analyses of neuronal proteins.
    Trimmer JS
    Journal of neuroscience methods 144.2 (2005 Jun 15): 165-73.

    IF References

    A primary culture system for biochemical analyses of neuronal proteins.
    Trimmer JS
    Journal of neuroscience methods 144.2 (2005 Jun 15): 165-73.

    WB References

    A primary culture system for biochemical analyses of neuronal proteins.
    Trimmer JS
    Journal of neuroscience methods 144.2 (2005 Jun 15): 165-73.

    IHC References

    A primary culture system for biochemical analyses of neuronal proteins.
    Trimmer JS
    Journal of neuroscience methods 144.2 (2005 Jun 15): 165-73.

    All References

    A primary culture system for biochemical analyses of neuronal proteins.
    Trimmer JS
    Journal of neuroscience methods 144.2 (2005 Jun 15): 165-73.

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