KC5-5E1

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$45.00 to $425.00
SKU: KC5-5E1
View product citations for antibody KC5-5E1 on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: GABA(A) receptor gamma-2 large intracellular loop
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: De Blas, Angel L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Bovine, Human, Rat
Depositors Institution: University of Connecticut
Antigen Molecular Weight: 54.1 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: GST-fusion protein of g2IL
Gene: GABRG2
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Uniprot ID: P18507 
Epitope Location or Sequence:
Entrez Gene ID: 2566 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2877066 
Additional Characterization:  
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
KC5-5E1 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product KC5-5E1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
  • IP References
  • All References
  • Initial Publication

    Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
    De Blas AL
    Journal of neurochemistry 64.3 (1995 Mar): 1305-11.

    WB References

    Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
    De Blas AL
    Journal of neurochemistry 64.3 (1995 Mar): 1305-11.

    IP References

    Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
    De Blas AL
    Journal of neurochemistry 64.3 (1995 Mar): 1305-11.

    All References

    Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
    De Blas AL
    Journal of neurochemistry 64.3 (1995 Mar): 1305-11.

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