Klotho KL-115

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$45.00
SKU: Klotho KL-115-s
View product citations for antibody Klotho KL-115 on CiteAb

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Available: 137

DSHB Data Sheet

Catalog Fields

Antigen: KLOTHO
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: RIgG2a, kappa light chain
Depositor: King, Gwendalyn
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: University of Alabama
Antigen Molecular Weight: Predicted: 116.1kDa Apparent: 130kDa
Depositors Notes: Antigen retrival with citrate buffer recommended.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant peptide sequence-GST fusion
Gene: KL
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3U-1
Epitope Mapped: Yes
Uniprot ID: Q9UEF7 
Epitope Location or Sequence: a.a. 55-261
Entrez Gene ID: 9365 
Immunogen Sequence: N-terminus of KL1 domain a.a.55-261
Antibody Registry ID: AB_2618099 
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: Slight reaction with mouse tissue in IHC after AR. Works in human only for Western blot. RRID:AB_2618099
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Klotho KL-115 was deposited to the DSHB by King, Gwendalyn (DSHB Hybridoma Product Klotho KL-115)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IF References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    WB References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IHC References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IP References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    FFPE References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    Epitope Map References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    All References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

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