Klotho KL-115

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$40.00
SKU: Klotho KL-115-s
View product citations for antibody Klotho KL-115 on CiteAb

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Available: 145

DSHB Data Sheet

Catalog Fields

Antigen: KLOTHO
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: King, Gwendalyn
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: University of Alabama
Positive Tested Species Reactivity: Human
Depositors Notes: Antigen retrival with citrate buffer recommended.
Antigen Molecular Weight: Predicted: 116.1kDa Apparent: 130kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: KL
Immunogen: Recombinant peptide sequence-GST fusion
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U-1
Epitope Location or Sequence: a.a. 55-261
Uniprot ID: Q9UEF7 
Immunogen Sequence: N-terminus of KL1 domain a.a.55-261
Entrez Gene ID: 9365 
Additional Characterization:  
Antibody Registry ID: AB_2618099 
Additional Information: Slight reaction with mouse tissue in IHC after AR. Works in human only for Western blot. RRID:AB_2618099
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Klotho KL-115 was deposited to the DSHB by King, Gwendalyn (DSHB Hybridoma Product Klotho KL-115)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IF References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    WB References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IHC References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IP References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    FFPE References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    Epitope Map References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    All References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

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