Klotho KL-115
Catalog Fields
Clone ID/Product Name: Klotho KL-115
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: KL
Ab Isotype: RIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_2618099
Uniprot ID: Q9UEF7
Entrez Gene ID: 9365
Clonality: Monoclonal
Immunogen: Recombinant peptide sequence-GST fusion
Clone:
Immunogen Sequence: N-terminus of KL1 domain a.a.55-261
Myeloma Strain: P3U-1
Epitope Mapped: Yes
Antigen Name: KLOTHO
Epitope Location or Sequence: a.a. 55-261
Alternate Antigen Name:
Deposit Date: 6/10/2014
Antigen Molecular Weight: Predicted: 116.1kDa Apparent: 130kDa
Depositor: King, Gwendalyn
Antigen Sequence:
Depositor Institution: University of Alabama
Antigen Species: Human
Depositor Notes: Antigen retrival with citrate buffer recommended.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: Slight reaction with mouse tissue in IHC after AR. Works in human only for Western blot. RRID:AB_2618099
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Klotho KL-115 was deposited to the DSHB by King, Gwendalyn (DSHB Hybridoma Product Klotho KL-115)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Development and characterization of monoclonal antibodies to detect klotho.
King GD
Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.