Klotho KL-115

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$40.00
SKU: Klotho KL-115-s
View product citations for antibody Klotho KL-115 on CiteAb

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Available: 145

DSHB Data Sheet

Catalog Fields

Antigen: KLOTHO
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: King, Gwendalyn
Isotype: RIgG2a, kappa light chain
Depositors Institution: University of Alabama
Host Species: rat
Depositors Notes: Antigen retrival with citrate buffer recommended.
Positive Tested Species Reactivity: Human
Gene: KL
Antigen Molecular Weight: Predicted: 116.1kDa Apparent: 130kDa
Clonality: Monoclonal
Immunogen: Recombinant peptide sequence-GST fusion
Epitope Mapped: Yes
Myeloma Strain: P3U-1
Epitope Location or Sequence: a.a. 55-261
Uniprot ID: Q9UEF7 
Immunogen Sequence: N-terminus of KL1 domain a.a.55-261
Entrez Gene ID: 9365 
Additional Information: Slight reaction with mouse tissue in IHC after AR. Works in human only for Western blot. RRID:AB_2618099
Antibody Registry ID: AB_2618099 
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Klotho KL-115 was deposited to the DSHB by King, Gwendalyn (DSHB Hybridoma Product Klotho KL-115)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IF References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    WB References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IHC References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    IP References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    FFPE References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    Epitope Map References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

    All References

    Development and characterization of monoclonal antibodies to detect klotho.
    King GD
    Monoclonal antibodies in immunodiagnosis and immunotherapy 33.6 (2014 Dec): 420-7.

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