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SKU: L21/32
View product citations for antibody L21/32 on CiteAb

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Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: GluA2/GluR2 glutamate receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Davis
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Does not cross-react with GluA1/GluR1, GluA3/GluR3 or GluA4/GluR4 (based on KO validation results).
Antigen Molecular Weight: 90 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: GRIA2
Immunogen: Cytoplasmic C-terminus fusion protein
Alternate Gene Names: GluA2; GluR2; gluR-B; GluR-K2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P19491 
Immunogen Sequence: a.a. 834-883
Entrez Gene ID: 29627 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
L21/32 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product L21/32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Rapid synthesis and synaptic insertion of GluR2 for mGluR-LTD in the ventral tegmental area.
    Lüscher C
    Science (New York, N.Y.) 317.5837 (2007 Jul 27): 530-3.

    All References

    Rapid synthesis and synaptic insertion of GluR2 for mGluR-LTD in the ventral tegmental area.
    Lüscher C
    Science (New York, N.Y.) 317.5837 (2007 Jul 27): 530-3.

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