L23/27

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SKU: L23/27

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: L23/27
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: KCNA3
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_2877316 
Uniprot ID: P15384 
RRID:  
Entrez Gene ID: 29731 
Clonality: Monoclonal
Immunogen: Cytoplasmic C-terminus synthetic peptide
Clone:
Immunogen Sequence: a.a. 485-506
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Kv1.3 K+ channel
Epitope Location or Sequence: extracellular domain
Alternate Antigen Name:
Deposit Date: 2/21/2019
Antigen Molecular Weight: 70 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Rat
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
L23/27 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product L23/27)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Kv1.3 channels in postganglionic sympathetic neurons: expression, function, and modulation.
    Damon DH
    American journal of physiology. Regulatory, integrative and comparative physiology 295.3 (2008 Sep): R733-40.

    IF References

    Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons.
    Kaczmarek LK
    The Journal of comparative neurology 518.16 (2010 Aug 15): 3205-20.

    WB References

    Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons.
    Kaczmarek LK
    The Journal of comparative neurology 518.16 (2010 Aug 15): 3205-20.

    IHC References

    Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons.
    Kaczmarek LK
    The Journal of comparative neurology 518.16 (2010 Aug 15): 3205-20.

    Epitope Map References

    Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons.
    Kaczmarek LK
    The Journal of comparative neurology 518.16 (2010 Aug 15): 3205-20.

    All References

    Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons.
    Kaczmarek LK
    The Journal of comparative neurology 518.16 (2010 Aug 15): 3205-20.

    Kv1.3 channels in postganglionic sympathetic neurons: expression, function, and modulation.
    Damon DH
    American journal of physiology. Regulatory, integrative and comparative physiology 295.3 (2008 Sep): R733-40.

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