LC1

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$40.00
SKU: LC1
View product citations for antibody LC1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin, hTPM3
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Hamster, Human
Depositors Notes: Recognizes Human non-muscle tropomyosin (hTM5), hamster (CHO) but not mouse.
Antigen Molecular Weight: Apparent: 32 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM3
Immunogen: Recombinant human tropomyosin isoform 5 and 4 fusion (hTM5/4) protein (N-terminal half of hTM5 and C-terminal half of hTM4).
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: First ten aa of the human TPM3 exon 1b product
Uniprot ID: P06753 
Immunogen Sequence: Partial protein
Entrez Gene ID: 7170 
Additional Characterization:  
Antibody Registry ID: AB_10572389 
Additional Information: Doesn't cross-react with rat or mouse tropomyosin.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LC1 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product LC1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    IF References

    Isoform sorting of tropomyosins.
    Gunning P
    Advances in experimental medicine and biology 644. (2008): 187-200.

    Antibody to tropomyosin isoform 5 and complement induce the lysis of colonocytes in ulcerative colitis.
    Das KM
    The American journal of gastroenterology 104.12 (2009 Dec): 2996-3003.

    Tropomyosin and caldesmon regulate cytokinesis speed and membrane stability during cell division.
    Lin JJ
    Archives of biochemistry and biophysics 456.2 (2006 Dec 15): 161-74.

    WB References

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5.
    Sung LA
    Archives of biochemistry and biophysics 378.1 (2000 Jun 1): 16-24.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5.
    Sung LA
    Archives of biochemistry and biophysics 378.1 (2000 Jun 1): 16-24.

    Isolation and sequencing of a novel tropomyosin isoform preferentially associated with colon cancer.
    Lin JJ
    Gastroenterology 123.1 (2002 Jul): 152-62.

    Tropomyosin isoform 5b is expressed in human erythrocytes: implications of tropomodulin-TM5 or tropomodulin-TM5b complexes in the protofilament and hexagonal organization of membrane skeletons.
    Mehrpouryan M
    Blood 95.4 (2000 Feb 15): 1473-80.

    Tropomyosin regulates cell migration during skin wound healing.
    O'Neill GM
    The Journal of investigative dermatology 133.5 (2013 May): 1330-9.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

    IP References
    Epitope Map References

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5.
    Sung LA
    Archives of biochemistry and biophysics 378.1 (2000 Jun 1): 16-24.

    All References

    Isoform sorting of tropomyosins.
    Gunning P
    Advances in experimental medicine and biology 644. (2008): 187-200.

    Antibody to tropomyosin isoform 5 and complement induce the lysis of colonocytes in ulcerative colitis.
    Das KM
    The American journal of gastroenterology 104.12 (2009 Dec): 2996-3003.

    Tropomyosin and caldesmon regulate cytokinesis speed and membrane stability during cell division.
    Lin JJ
    Archives of biochemistry and biophysics 456.2 (2006 Dec 15): 161-74.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5.
    Sung LA
    Archives of biochemistry and biophysics 378.1 (2000 Jun 1): 16-24.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Isolation and sequencing of a novel tropomyosin isoform preferentially associated with colon cancer.
    Lin JJ
    Gastroenterology 123.1 (2002 Jul): 152-62.

    Tropomyosin isoform 5b is expressed in human erythrocytes: implications of tropomodulin-TM5 or tropomodulin-TM5b complexes in the protofilament and hexagonal organization of membrane skeletons.
    Mehrpouryan M
    Blood 95.4 (2000 Feb 15): 1473-80.

    Tropomyosin regulates cell migration during skin wound healing.
    O'Neill GM
    The Journal of investigative dermatology 133.5 (2013 May): 1330-9.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

    Mapping the tropomyosin isoform 5 binding site on human erythrocyte tropomodulin: further insights into E-Tmod/TM5 interaction.
    Sung LA
    Archives of biochemistry and biophysics 444.2 (2005 Dec 15): 130-8.

    Tropomyosin isoforms in nonmuscle cells.
    Lin JL
    International review of cytology 170. (1997): 1-38.

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