LC24

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$40.00
SKU: LC24
View product citations for antibody LC24 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin, hTPM4
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Human, Rodent
Depositors Notes: Recognizes Human (hTM4) and rodent non-muscle tropomyosin.
Antigen Molecular Weight: Apparent: 36 and 30 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM4
Immunogen: Recombinant human tropomyosin isoform 5 and 4 fusion (hTM5/4) protein (N-terminal half of hTM5 and C-terminal half of hTM4).
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: C-terminal half of hTm4 (TPM4 exon 9d)
Uniprot ID: P67936 
Immunogen Sequence: Partial protein
Entrez Gene ID: 7171 
Additional Characterization:  
Antibody Registry ID: AB_10573119 
Additional Information: Cross reacts with Tm1 from TPM2.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LC24 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product LC24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    IF References

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Human tropomyosin isoforms in the regulation of cytoskeleton functions.
    McCrae KR
    Advances in experimental medicine and biology 644. (2008): 201-22.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    A high molecular mass non-muscle tropomyosin isoform stimulates retrograde organelle transport.
    Wang YL
    Journal of cell science 109 ( Pt 5). (1996 May): 981-9.

    WB References

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Changes in protein profiles during course of experimental glomerulonephritis.
    Budisavljevic MN
    American journal of physiology. Renal physiology 296.1 (2009 Jan): F186-93.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    Alterations in tropomyosin isoform expression in human transitional cell carcinoma of the urinary bladder.
    Helfman DM
    International journal of cancer 110.3 (2004 Jun 20): 368-73.

    Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments.
    Molitoris BA
    American journal of physiology. Renal physiology 286.5 (2004 May): F988-96.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

    IHC References
    FACS References

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    Epitope Map References

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    All References

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Human tropomyosin isoforms in the regulation of cytoskeleton functions.
    McCrae KR
    Advances in experimental medicine and biology 644. (2008): 201-22.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    A high molecular mass non-muscle tropomyosin isoform stimulates retrograde organelle transport.
    Wang YL
    Journal of cell science 109 ( Pt 5). (1996 May): 981-9.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Changes in protein profiles during course of experimental glomerulonephritis.
    Budisavljevic MN
    American journal of physiology. Renal physiology 296.1 (2009 Jan): F186-93.

    Alterations in tropomyosin isoform expression in human transitional cell carcinoma of the urinary bladder.
    Helfman DM
    International journal of cancer 110.3 (2004 Jun 20): 368-73.

    Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments.
    Molitoris BA
    American journal of physiology. Renal physiology 286.5 (2004 May): F988-96.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    Tropomyosin isoforms in nonmuscle cells.
    Lin JL
    International review of cytology 170. (1997): 1-38.

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