LM2/1.6.11

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SKU: LM2/1.6.11

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: LM2/1.6.11
Available to For-Profits: Yes
Alternate Antibody Name: LM2/1
Gene Symbol: ITGAM
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528135 
Uniprot ID: P11215 
RRID:  
Entrez Gene ID: 3684 
Clonality: Monoclonal
Immunogen: CD11b immunoprecipitated from Human granulocyte lysates + boost with Human live granulocytes.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: Yes
Antigen Name: CD11b (Integrin alpha-M, Mac-1 alpha chain), human
Epitope Location or Sequence: I domain (aa 148-332)
Alternate Antigen Name:
Deposit Date: 4/4/1989
Antigen Molecular Weight: Predicted: 127 kDa; Apparent: 170 kDa
Depositor: Springer, T.A.
Antigen Sequence:
Depositor Institution: Center for Blood Research, Boston
Antigen Species: Human
Depositor Notes: Does not cross react with mouse. Does not block iC3B rosetting. Binds to granulocytes, monocytes, macrophages, CD5+ B cells, hairy cell leukemia cells. Doesn't work well for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Mac-1 is a macrophage differentiation antigen and a marker for microglia.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LM2/1.6.11 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product LM2/1.6.11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.

    IF References
    WB References

    Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.

    IHC References

    Cytokines, chemokines, and cytokine receptors in human microglia.
    Kim SU
    Journal of neuroscience research 69.1 (2002 Jul 1): 94-103.

    IP References
    FB References

    Antibodies to CD18 influence neutrophil migration through extracellular matrix.
    Parkhurst MR
    Journal of leukocyte biology 65.3 (1999 Mar): 356-63.

    Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.

    Constitutive and induced phosphorylation of the alpha- and beta-chains of the CD11/CD18 leukocyte integrin family. Relationship to adhesion-dependent functions.
    Winchester R
    Journal of immunology (Baltimore, Md. : 1950) 144.1 (1990 Jan 1): 191-7.

    Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.1 (1986 Jul 1): 15-27.

    Complement opsonization is required for presentation of immune complexes by resting peripheral blood B cells.
    Karp DR
    Journal of immunology (Baltimore, Md. : 1950) 161.12 (1998 Dec 15): 6537-43.

    Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells.
    Van Furth R
    Immunology 74.4 (1991 Dec): 661-9.

    Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells.
    Ehlers MR
    Infection and immunity 64.12 (1996 Dec): 5373-83.

    The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands.
    Springer TA
    The Journal of cell biology 120.4 (1993 Feb): 1031-43.

    A quantitative radiometric assay to measure mammalian cell binding to hyphae of Candida albicans.
    Mathews HL
    Journal of immunological methods 165.1 (1993 Sep 27): 113-9.

    FACS References

    Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.

    The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands.
    Springer TA
    The Journal of cell biology 120.4 (1993 Feb): 1031-43.

    Epitope Map References
    All References

    Cytokines, chemokines, and cytokine receptors in human microglia.
    Kim SU
    Journal of neuroscience research 69.1 (2002 Jul 1): 94-103.

    The role of lipooligosaccharide in Neisseria gonorrhoeae pathogenesis of cervical epithelia: lipid A serves as a C3 acceptor molecule.
    Apicella MA
    Cellular microbiology 4.9 (2002 Sep): 585-98.

    Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.

    Constitutive and induced phosphorylation of the alpha- and beta-chains of the CD11/CD18 leukocyte integrin family. Relationship to adhesion-dependent functions.
    Winchester R
    Journal of immunology (Baltimore, Md. : 1950) 144.1 (1990 Jan 1): 191-7.

    Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells.
    Ehlers MR
    Infection and immunity 64.12 (1996 Dec): 5373-83.

    The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands.
    Springer TA
    The Journal of cell biology 120.4 (1993 Feb): 1031-43.

    Integrin alphaMbeta2 orchestrates and accelerates plasminogen activation and fibrinolysis by neutrophils.
    Plow EF
    The Journal of biological chemistry 279.17 (2004 Apr 23): 18063-72.

    Distinct ligand binding sites in the I domain of integrin alpha M beta 2 that differentially affect a divalent cation-dependent conformation.
    Bajt ML
    The Journal of biological chemistry 270.43 (1995 Oct 27): 25866-71.

    Antibodies to CD18 influence neutrophil migration through extracellular matrix.
    Parkhurst MR
    Journal of leukocyte biology 65.3 (1999 Mar): 356-63.

    Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.1 (1986 Jul 1): 15-27.

    Complement opsonization is required for presentation of immune complexes by resting peripheral blood B cells.
    Karp DR
    Journal of immunology (Baltimore, Md. : 1950) 161.12 (1998 Dec 15): 6537-43.

    Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells.
    Van Furth R
    Immunology 74.4 (1991 Dec): 661-9.

    A quantitative radiometric assay to measure mammalian cell binding to hyphae of Candida albicans.
    Mathews HL
    Journal of immunological methods 165.1 (1993 Sep 27): 113-9.

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