LM2/1.6.11
Catalog Fields
Antigen: CD11b (Integrin alpha-M, Mac-1 alpha chain), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Springer, T.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Center for Blood Research, Boston
Positive Tested Species Reactivity: Human
Depositors Notes: Does not cross react with mouse. Does not block iC3B rosetting. Binds to granulocytes, monocytes, macrophages, CD5+ B cells, hairy cell leukemia cells. Doesn't work well for western blotting.
Predicted Species Reactivity:
Gene: ITGAM
Immunogen: CD11b immunoprecipitated from Human granulocyte lysates + boost with Human live granulocytes.
Alternate Gene Names: MAC-1, MO1A, CR3
Alternate Antibody Name: LM2/1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence: I domain (aa 148-332)
Uniprot ID: P11215
Immunogen Sequence: Full length protein
Entrez Gene ID: 3684
Antibody Registry ID: AB_528135
Additional Information: Mac-1 is a macrophage differentiation antigen and a marker for microglia.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LM2/1.6.11 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product LM2/1.6.11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
Springer TA
Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.