LM2/1.6.11
Catalog Fields
Product Name/ID: LM2/1.6.11
Available to For-Profits: Yes
Alternate Antibody Name: LM2/1
Gene Name: ITGAM
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 3684
Clonality: Monoclonal
Immunogen: CD11b immunoprecipitated from Human granulocyte lysates + boost with Human live granulocytes.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: Yes
Antigen Name: CD11b (Integrin alpha-M, Mac-1 alpha chain), human
Epitope Location or Sequence: I domain (aa 148-332)
Alternate Antigen Name:
Deposit Date: 4/4/1989
Antigen Molecular Weight: Predicted: 127 kDa; Apparent: 170 kDa
Depositor: Springer, T.A.
Antigen Sequence:
Depositor Institution: Center for Blood Research, Boston
Antigen Species: Human
Depositor Notes: Does not cross react with mouse. Does not block iC3B rosetting. Binds to granulocytes, monocytes, macrophages, CD5+ B cells, hairy cell leukemia cells. Doesn't work well for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Mac-1 is a macrophage differentiation antigen and a marker for microglia.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LM2/1.6.11 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product LM2/1.6.11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
Springer TA
Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.