LM2/1.6.11
Catalog Fields
Antigen: CD11b (Integrin alpha-M, Mac-1 alpha chain), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Springer, T.A.
Isotype: MIgG1
Depositors Institution: Center for Blood Research, Boston
Host Species: mouse
Depositors Notes: Does not cross react with mouse. Does not block iC3B rosetting. Binds to granulocytes, monocytes, macrophages, CD5+ B cells, hairy cell leukemia cells. Doesn't work well for western blotting.
Positive Tested Species Reactivity: Human
Gene: ITGAM
Antigen Molecular Weight: Predicted: 127 kDa; Apparent: 170 kDa
Alternate Gene Names: MAC-1, MO1A, CR3
Immunogen: CD11b immunoprecipitated from Human granulocyte lysates + boost with Human live granulocytes.
Clonality: Monoclonal
Alternate Antibody Name: LM2/1
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence: I domain (aa 148-332)
Uniprot ID: P11215
Immunogen Sequence: Full length protein
Entrez Gene ID: 3684
Additional Information: Mac-1 is a macrophage differentiation antigen and a marker for microglia.
Antibody Registry ID: AB_528135
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LM2/1.6.11 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product LM2/1.6.11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
Springer TA
Journal of immunology (Baltimore, Md. : 1950) 137.9 (1986 Nov 1): 2891-900.