LOS 3F11
Catalog Fields
Product Name/ID: LOS 3F11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_2617191
Clonality: Monoclonal
Immunogen: Heat-killed gonococcal strain 4505 cells (LPS serotype Gc3)
Clone:
Immunogen Sequence: Lipopolysaccharide
Myeloma Strain: P3x63Ag8
Epitope Mapped: Yes
Antigen Name: lipooligosaccharide (LOS)
Epitope Location or Sequence: LOS Gal beta1-4GlcNAc
Alternate Antigen Name:
Deposit Date: 3/28/2013
Antigen Molecular Weight: 4.5 kDa
Depositor: Apicella, M.A.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Neisseria gonorrhoeae
Depositor Notes: Immunogen species is Neisseria gonorrhoeae strain 1291. 3F11 recognizes a site common to gonococcal LPS and is partially shared with meningococcal LPS. Sialylation of LOS obscures the 3F11 epitope. Therefore, for IHC or IF, remove sialic acid residues from LOS pre-treatment with neuraminidase is required.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Neisseria gonorrhoeae, Neisseria meningitidis
Additional Information: RRID: AB_2617191
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LOS 3F11 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product LOS 3F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
18 References
Initial Publication
Monoclonal antibody analysis of lipopolysaccharide from Neisseria gonorrhoeae and Neisseria meningitidis.
Roberts DE
Infection and immunity 34.3 (1981 Dec): 751-6.