LOS 3F11

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$40.00
SKU: LOS 3F11
View product citations for antibody LOS 3F11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: lipooligosaccharide (LOS)
Hybridoma Cells Available: Yes
Antigen Species: Neisseria gonorrhoeae
Depositor: Apicella, M.A.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Neisseria gonorrhoeae, Neisseria meningitidis
Depositors Notes: Immunogen species is Neisseria gonorrhoeae strain 1291. 3F11 recognizes a site common to gonococcal LPS and is partially shared with meningococcal LPS. Sialylation of LOS obscures the 3F11 epitope. Therefore, for IHC or IF, remove sialic acid residues from LOS pre-treatment with neuraminidase is required.
Antigen Molecular Weight: 4.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Heat-killed gonococcal strain 4505 cells (LPS serotype Gc3)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8
Epitope Location or Sequence: LOS Gal beta1-4GlcNAc
Uniprot ID:  
Immunogen Sequence: Lipopolysaccharide
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2617191 
Additional Information: RRID: AB_2617191
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LOS 3F11 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product LOS 3F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Lipooligosaccharide biosynthesis in pathogenic Neisseria. Cloning, identification, and characterization of the phosphoglucomutase gene.
    Apicella MA
    The Journal of biological chemistry 269.15 (1994 Apr 15): 11162-9.

    Molecular analysis of lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.
    Stein DC
    Infection and immunity 57.9 (1989 Sep): 2847-52.

    The relative roles of factor H binding protein, neisserial surface protein A, and lipooligosaccharide sialylation in regulation of the alternative pathway of complement on meningococci.
    Ram S
    Journal of immunology (Baltimore, Md. : 1950) 188.10 (2012 May 15): 5063-72.

    Structural analysis of lipooligosaccharide produced by Neisseria gonorrhoeae, strain MS11mk (variant A): a precursor for a gonococcal lipooligosaccharide associated with virulence.
    Yamasaki R
    Biochemistry 31.51 (1992 Dec 29): 12760-8.

    Sialylation of lipooligosaccharides promotes biofilm formation by nontypeable Haemophilus influenzae.
    VonCannon J
    Infection and immunity 72.1 (2004 Jan): 106-13.

    Nontypeable Haemophilus influenzae Lipooligosaccharide Expresses a Terminal Ketodeoxyoctanoate <i>In Vivo</i>, Which Can Be Used as a Target for Bactericidal Antibody.
    Jennings MP
    mBio 9.4 (2018 Jul 31): .

    Comparative Analyses of the Lipooligosaccharides from Nontypeable Haemophilus influenzae and Haemophilus haemolyticus Show Differences in Sialic Acid and Phosphorylcholine Modifications.
    Apicella MA
    Infection and immunity 84.3 (2016 Jan 4): 765-74.

    Desialylation of Neisseria gonorrhoeae Lipooligosaccharide by Cervicovaginal Microbiome Sialidases: The Potential for Enhancing Infectivity in Men.
    Apicella MA
    The Journal of infectious diseases 214.11 (2016 Dec 1): 1621-1628.

    Phase-Variable Heptose I Glycan Extensions Modulate Efficacy of 2C7 Vaccine Antibody Directed against Neisseria gonorrhoeae Lipooligosaccharide.
    Ram S
    Journal of immunology (Baltimore, Md. : 1950) 196.11 (2016 Jun 1): 4576-86.

    ELISA References
    FACS References
    Epitope Map References
    All References

    Activation of complement by mannose-binding lectin on isogenic mutants of Neisseria meningitidis serogroup B.
    Klein NJ
    Journal of immunology (Baltimore, Md. : 1950) 160.3 (1998 Feb 1): 1346-53.

    Receptor-mediated endocytosis of Neisseria gonorrhoeae into primary human urethral epithelial cells: the role of the asialoglycoprotein receptor.
    Apicella MA
    Molecular microbiology 42.3 (2001 Nov): 659-72.

    Nontypeable Haemophilus influenzae Lipooligosaccharide Expresses a Terminal Ketodeoxyoctanoate <i>In Vivo</i>, Which Can Be Used as a Target for Bactericidal Antibody.
    Jennings MP
    mBio 9.4 (2018 Jul 31): .

    Desialylation of Neisseria gonorrhoeae Lipooligosaccharide by Cervicovaginal Microbiome Sialidases: The Potential for Enhancing Infectivity in Men.
    Apicella MA
    The Journal of infectious diseases 214.11 (2016 Dec 1): 1621-1628.

    Lipooligosaccharide biosynthesis in pathogenic Neisseria. Cloning, identification, and characterization of the phosphoglucomutase gene.
    Apicella MA
    The Journal of biological chemistry 269.15 (1994 Apr 15): 11162-9.

    Molecular analysis of lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.
    Stein DC
    Infection and immunity 57.9 (1989 Sep): 2847-52.

    The relative roles of factor H binding protein, neisserial surface protein A, and lipooligosaccharide sialylation in regulation of the alternative pathway of complement on meningococci.
    Ram S
    Journal of immunology (Baltimore, Md. : 1950) 188.10 (2012 May 15): 5063-72.

    Structural analysis of lipooligosaccharide produced by Neisseria gonorrhoeae, strain MS11mk (variant A): a precursor for a gonococcal lipooligosaccharide associated with virulence.
    Yamasaki R
    Biochemistry 31.51 (1992 Dec 29): 12760-8.

    Sialylation of lipooligosaccharides promotes biofilm formation by nontypeable Haemophilus influenzae.
    VonCannon J
    Infection and immunity 72.1 (2004 Jan): 106-13.

    Comparative Analyses of the Lipooligosaccharides from Nontypeable Haemophilus influenzae and Haemophilus haemolyticus Show Differences in Sialic Acid and Phosphorylcholine Modifications.
    Apicella MA
    Infection and immunity 84.3 (2016 Jan 4): 765-74.

    Phase-Variable Heptose I Glycan Extensions Modulate Efficacy of 2C7 Vaccine Antibody Directed against Neisseria gonorrhoeae Lipooligosaccharide.
    Ram S
    Journal of immunology (Baltimore, Md. : 1950) 196.11 (2016 Jun 1): 4576-86.

    Monoclonal antibody analysis of lipopolysaccharide from Neisseria gonorrhoeae and Neisseria meningitidis.
    Roberts DE
    Infection and immunity 34.3 (1981 Dec): 751-6.

    Variation in phenotypic expression of the Opa outer membrane protein and lipooligosaccharide of Neisseria meningitidis serogroup C causing periorbital cellulitis and bacteremia.
    Baker CJ
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 16.4 (1993 Apr): 523-7.

    Targeting Lipooligosaccharide (LOS) for a Gonococcal Vaccine.
    Rice PA
    Frontiers in immunology 10. (2019): 321.

    Role of Gonococcal Neisserial Surface Protein A (NspA) in Serum Resistance and Comparison of Its Factor H Binding Properties with Those of Its Meningococcal Counterpart.
    Ram S
    Infection and immunity 87.2 (2019 Feb): .

    A Novel Sialylation Site on Neisseria gonorrhoeae Lipooligosaccharide Links Heptose II Lactose Expression with Pathogenicity.
    Rice PA
    Infection and immunity 86.8 (2018 Aug): .

    A Novel Factor H-Fc Chimeric Immunotherapeutic Molecule against Neisseria gonorrhoeae.
    Ram S
    Journal of immunology (Baltimore, Md. : 1950) 196.4 (2016 Feb 15): 1732-40.

    Selection and immunochemical analysis of lipooligosaccharide mutants of Neisseria gonorrhoeae.
    Apicella MA
    Infection and immunity 56.2 (1988 Feb): 499-504.

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