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DSHB Data Sheet

Catalog Fields

Product Name/ID: LOS 6B4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2617193 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Heat-killed gonococcal strain 4505 cells (LPS serotype Gc3)
Immunogen Sequence: Lipopolysaccharide
Myeloma Strain: P3x63Ag8
Epitope Mapped: Yes
Antigen Name: lipooligosaccharide (LOS)
Epitope Location or Sequence: LOS Gal beta1-4GlcNAc
Alternate Antigen Name:
Deposit Date: 3/28/2013
Antigen Molecular Weight:
Depositor: Apicella, M.A.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Neisseria gonorrhoeae
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Haemophilus ducreyi, Neisseria gonorrhoeae
Additional Information: RRID: AB_2617193
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LOS 6B4 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product LOS 6B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ELISA References
    Epitope Map References
    All References

    Modification by sialic acid of Neisseria gonorrhoeae lipooligosaccharide epitope expression in human urethral exudates: an immunoelectron microscopic analysis.
    Rice PA
    The Journal of infectious diseases 162.2 (1990 Aug): 506-12.

    The msbB mutant of Neisseria meningitidis strain NMB has a defect in lipooligosaccharide assembly and transport to the outer membrane.
    Apicella MA
    Infection and immunity 71.2 (2003 Feb): 647-55.

    Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi.
    Spinola SM
    Infection and immunity 59.8 (1991 Aug): 2601-8.

    Biofilm Formation by Neisseria gonorrhoeae.
    Apicella MA
    Infection and immunity 73.4 (2005 Apr): 1964-70.

    Desialylation of Neisseria gonorrhoeae Lipooligosaccharide by Cervicovaginal Microbiome Sialidases: The Potential for Enhancing Infectivity in Men.
    Apicella MA
    The Journal of infectious diseases 214.11 (2016 Dec 1): 1621-1628.

    Nontypeable Haemophilus influenzae Lipooligosaccharide Expresses a Terminal Ketodeoxyoctanoate In Vivo, Which Can Be Used as a Target for Bactericidal Antibody.
    Jennings MP
    mBio 9.4 (2018 Jul 31): .

    Gonococcal lipooligosaccharide is a ligand for the asialoglycoprotein receptor on human sperm.
    Blake MS
    Molecular microbiology 36.5 (2000 Jun): 1059-70.

    Selection and immunochemical analysis of lipooligosaccharide mutants of Neisseria gonorrhoeae.
    Apicella MA
    Infection and immunity 56.2 (1988 Feb): 499-504.

    Interaction of the gonococcal porin P.IB with G- and F-actin.
    Rubenstein PA
    Biochemistry 39.29 (2000 Jul 25): 8638-47.

    Monoclonal antibody analysis of lipopolysaccharide from Neisseria gonorrhoeae and Neisseria meningitidis.
    Roberts DE
    Infection and immunity 34.3 (1981 Dec): 751-6.

    Multiple gonococcal opacity proteins are expressed during experimental urethral infection in the male.
    Cannon JG
    The Journal of experimental medicine 179.3 (1994 Mar 1): 911-20.

    Antigenic diversity of Haemophilus somnus lipooligosaccharide: phase-variable accessibility of the phosphorylcholine epitope.
    Inzana TJ
    Journal of clinical microbiology 38.12 (2000 Dec): 4412-9.

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