LOS 6B7

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$40.00
SKU: LOS 6B7
View product citations for antibody LOS 6B7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: lipooligosaccharide (LOS)
Hybridoma Cells Available: Yes
Antigen Species: Neisseria meningitidis
Depositor: Apicella, M.A.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Neisseria gonorrhoeae, Neisseria meningitidis
Depositors Notes: LOS 6B7 was generated against N. meningitidis group A strain A1, which has an L11 LOS.
Antigen Molecular Weight: 3.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Heat-killed N. meningitidis group A strain A
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8
Epitope Location or Sequence: LOS Gal beta1-4GlcNAc
Uniprot ID:  
Immunogen Sequence: Lipopolysaccharide
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2827392 
Additional Information: RRID:AB_2827392
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LOS 6B7 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product LOS 6B7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    WB References
    IHC References
    ELISA References
    Epitope Map References

    Lipooligosaccharide epitopes shared among gram-negative non-enteric mucosal pathogens.
    Apicella MA
    Microbial pathogenesis 8.5 (1990 May): 353-62.

    All References

    Modification by sialic acid of Neisseria gonorrhoeae lipooligosaccharide epitope expression in human urethral exudates: an immunoelectron microscopic analysis.
    Rice PA
    The Journal of infectious diseases 162.2 (1990 Aug): 506-12.

    Lipooligosaccharide epitopes shared among gram-negative non-enteric mucosal pathogens.
    Apicella MA
    Microbial pathogenesis 8.5 (1990 May): 353-62.

    Measurement of the human immune response to meningococcal lipooligosaccharide antigens by using serum to inhibit monoclonal antibody binding to purified lipooligosaccharide.
    Griffiss JM
    Infection and immunity 58.7 (1990 Jul): 2204-13.

    Selection and immunochemical analysis of lipooligosaccharide mutants of Neisseria gonorrhoeae.
    Apicella MA
    Infection and immunity 56.2 (1988 Feb): 499-504.

    In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation.
    Apicella MA
    The Journal of experimental medicine 171.5 (1990 May 1): 1649-64.

    Sialylation and human neutrophil killing of group C Neisseria meningitidis.
    Mandrell RE
    The Journal of infectious diseases 166.5 (1992 Nov): 1079-88.

    The immune response of children to meningococcal lipooligosaccharides during disseminated disease is directed primarily against two monoclonal antibody-defined epitopes.
    Griffiss JM
    The Journal of infectious diseases 167.4 (1993 Apr): 966-70.

    Enzymatically deacylated Neisseria lipopolysaccharide (LPS) inhibits murine splenocyte mitogenesis induced by LPS.
    Munford RS
    Infection and immunity 59.6 (1991 Jun): 1881-7.

    Vaccine potential of meningococcal FrpB: studies on surface exposure and functional attributes of common epitopes.
    Borriello SP
    Vaccine 12.6 (1994 May): 535-41.

    Construction of isogenic gonococci with variable porin structure: effects on susceptibility to human serum and antibiotics.
    Sparling PF
    Molecular microbiology 4.6 (1990 Jun): 1009-18.

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