LOS 6E4

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$40.00
SKU: LOS 6E4
View product citations for antibody LOS 6E4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: lipooligosaccharide (LOS)
Hybridoma Cells Available: Yes
Antigen Species: Haemophilus influenzae
Depositor: Apicella, M.A.
Isotype: MIgG3, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Haemophilus influenzae, Neisseria gonorrhoeae, Salmonella minnesota
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Heat-killed Haemophilus influenzae strain Rd/b+
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8
Epitope Location or Sequence: ketodeoxyoctanoate
Uniprot ID:  
Immunogen Sequence: Lipopolysaccharide
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2617194 
Additional Information: RRID: AB_2617194
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LOS 6E4 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product LOS 6E4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Lipooligosaccharide epitopes shared among gram-negative non-enteric mucosal pathogens.
    Apicella MA
    Microbial pathogenesis 8.5 (1990 May): 353-62.

    IF References
    WB References

    Lipooligosaccharide epitopes shared among gram-negative non-enteric mucosal pathogens.
    Apicella MA
    Microbial pathogenesis 8.5 (1990 May): 353-62.

    ELISA References
    FACS References
    Epitope Map References
    All References

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