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$45.00 to $425.00
SKU: Lan2-3a/Lan2-3
View product citations for antibody Lan2-3a/Lan2-3 on CiteAb

In Stock

Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: CNS, leech
Hybridoma Cells Available: Yes
Antigen Species: leech
Depositor: Zipser, B.
Isotype: MIgG3
Antigen Sequence:
Host Species: mouse
Depositors Institution: Michigan State University
Positive Tested Species Reactivity: Blackworm, Leech
Depositors Notes: Antigen chemical properties: galactose-containing N-linked glycan on homologues of L1CAM and NCAM. Characterization: by experimental manipulation using enzymes and neoglycoprotein. Functional effects: promotes fasciculation of small subset of leech chemodetector neurons. IHC note: neuroepithelial glycan.
Antigen Molecular Weight: 130 kDa leech glycoprotein
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: paraformaldehyde-fixed minced CNS
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP-2
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10804047 
Additional Information:
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Lan2-3a/Lan2-3 was deposited to the DSHB by Zipser, B. (DSHB Hybridoma Product Lan2-3a/Lan2-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibodies distinguish identifiable neurones in the leech.
    McKay R
    Nature 289.5798 (1981 Feb 12): 549-54.

    All References

    Monoclonal antibodies distinguish identifiable neurones in the leech.
    McKay R
    Nature 289.5798 (1981 Feb 12): 549-54.

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