Laz6-189/Mannitou Ab

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$45.00 to $425.00
SKU: Laz6-189/Mannitou Ab
View product citations for antibody Laz6-189/Mannitou Ab on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: Trimannosidic paucimannose (mannosidic N-glycoepitope)
Available to For-Profits: Yes
Antigen Species: Leech
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Zipser, Birgit
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Blackworm, Capitellids, Erythrina Cristagalli, Human, Leech, Mouse, Rat, Seahorse
Depositors Institution: Michigan State University
Antigen Molecular Weight: 130 kDa
Depositors Notes: This antibody recognizes neural progenitor cells in mouse embryos, human glioblastoma cells, human macrophages and adult pancreatic stem cells.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Leech CNS
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP-2
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence: Carbohydrate
Antibody Registry ID: AB_10804297 
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: J Carbohydr Chem (2012) 31:504-518 ; RRID:AB_10804297
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Laz6-189/Mannitou Ab was deposited to the DSHB by Zipser, Birgit (DSHB Hybridoma Product Laz6-189/Mannitou Ab)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    FFPE References
    All References

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