Laz6-189/Mannitou Ab

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SKU: Laz6-189/Mannitou Ab
View product citations for antibody Laz6-189/Mannitou Ab on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Trimannosidic paucimannose (mannosidic N-glycoepitope)
Hybridoma Cells Available: Yes
Antigen Species: Leech
Depositor: Zipser, Birgit
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Michigan State University
Positive Tested Species Reactivity: Blackworm, Capitellids, Erythrina Cristagalli, Human, Leech, Mouse, Rat, Seahorse
Depositors Notes: This antibody recognizes neural progenitor cells in mouse embryos, human glioblastoma cells, human macrophages and adult pancreatic stem cells.
Antigen Molecular Weight: 130 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: Leech CNS
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP-2
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Carbohydrate
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10804297 
Additional Information: J Carbohydr Chem (2012) 31:504-518 ; RRID:AB_10804297
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Laz6-189/Mannitou Ab was deposited to the DSHB by Zipser, Birgit (DSHB Hybridoma Product Laz6-189/Mannitou Ab)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    FFPE References
    All References

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