Lost

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$40.00
SKU: Lost
View product citations for antibody Lost on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Lost protein; LD30155p
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Gavis, E.R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University - Princeton, NJ
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 59.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: lost
Immunogen: MBP-Lost fusion protein
Alternate Gene Names: Dmel\CG14648, Growl
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: 1D11
Epitope Location or Sequence:
Uniprot ID: Q9VN21 
Immunogen Sequence: Full length protein
Entrez Gene ID: 40559 
Additional Characterization:  
Antibody Registry ID: AB_2618045 
Additional Information: RRID:AB_2618045. FBgn0263594.
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Lost was deposited to the DSHB by Gavis, E.R. (DSHB Hybridoma Product Lost)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    A late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin.
    Gavis ER
    Development (Cambridge, England) 138.16 (2011 Aug): 3431-40.

    WB References

    A late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin.
    Gavis ER
    Development (Cambridge, England) 138.16 (2011 Aug): 3431-40.

    IP References

    A late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin.
    Gavis ER
    Development (Cambridge, England) 138.16 (2011 Aug): 3431-40.

    ELISA References

    A late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin.
    Gavis ER
    Development (Cambridge, England) 138.16 (2011 Aug): 3431-40.

    All References

    A late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin.
    Gavis ER
    Development (Cambridge, England) 138.16 (2011 Aug): 3431-40.

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