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SKU: M17/
View product citations for antibody M17/ on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD11a (Integrin alpha-L, LFA-1 alpha subunit), murine
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Springer, T.A.
Isotype: RIgG2a, kappa light chain
Depositors Institution: Center for Blood Research, Boston
Host Species: rat
Depositors Notes: Does not cross react with human antigen. Binds to all leukocytes except resident peritoneal macrophages. Doesn't appear to work for western blotting.
Positive Tested Species Reactivity: Mouse
Gene: Itgal
Antigen Molecular Weight: Predicted: 128 kDa; Apparent: 180 kDa
Alternate Gene Names: LFA-1, Ly-15
Immunogen: Anti-P815 cytotoxic T-lymphocytes from C57BL/6J mice.
Clonality: Monoclonal
Alternate Antibody Name: M17/4
Epitope Mapped: Yes
Myeloma Strain: NS1 or P3X63Ag8.6.5.3
Epitope Location or Sequence: I domain (aa 248-303)
Uniprot ID: P24063 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16408 
Antibody Registry ID: AB_2280611 
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M17/ was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product M17/
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IP References
    FB References
    FACS References
    Epitope Map References
    All References

    Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship.
    Springer TA
    Cellular immunology 73.1 (1982 Oct): 1-11.

    Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct.
    Roos E
    Journal of leukocyte biology 60.6 (1996 Dec): 758-65.

    Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor.
    Springer TA
    The Journal of biological chemistry 257.20 (1982 Oct 25): 12412-8.

    Efficiency of antibodies directed against adhesion molecules to prolong skin graft survival in mice.
    Figdor CG
    Transplantation proceedings 26.2 (1994 Apr): 401-3.

    Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
    Schreiber RD
    The Journal of experimental medicine 156.4 (1982 Oct 1): 1000-9.

    Protein tyrosine kinase p56-Lck regulates lymphocyte function-associated 1 adhesion molecule expression, granule exocytosis, and cytolytic effector function in a cloned T cell.
    Tachman R [corrected to Taichman R]
    The Journal of experimental medicine 180.3 (1994 Sep 1): 1115-27.

    A statin-based inhibitor of lymphocyte function antigen-1 protects against ischemia/reperfusion-induced leukocyte adhesion in the colon.
    Thorlacius H
    British journal of pharmacology 140.2 (2003 Sep): 395-401.

    Nonopsonic and opsonic association of Mycobacterium tuberculosis with resident alveolar macrophages is inefficient.
    Speert DP
    Journal of immunology (Baltimore, Md. : 1950) 160.11 (1998 Jun 1): 5514-21.

    An in vivo animal model of gene therapy for leukocyte adhesion deficiency.
    Wilson JM
    The Journal of clinical investigation 88.4 (1991 Oct): 1412-7.

    A binding interface on the I domain of lymphocyte function-associated antigen-1 (LFA-1) required for specific interaction with intercellular adhesion molecule 1 (ICAM-1).
    Springer TA
    The Journal of biological chemistry 270.32 (1995 Aug 11): 19008-16.

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