MANCHO10(9E10)

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$40.00
SKU: MANCHO10(9E10)
View product citations for antibody MANCHO10(9E10) on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: utrophin
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Species specificity note: human, mouse (not fish or xenopus). Immunoblotting note: stains 400 kDa band in HeLa total protein extract. IHC note: sarcolemma of fibers in Duchenne muscular dystrophy; blood vessels, nerves and neuromuscular junctions.
Antigen Molecular Weight: 155 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: human protein, utrophin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: epitope(aa) 3164-3184 by phage-display
Entrez Gene ID:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANCHO10(9E10) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANCHO10(9E10))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    An epitope structure for the C-terminal domain of dystrophin and utrophin.
    Nguyen thi Man
    Biochemistry 37.31 (1998 Aug 4): 11117-27.

    All References

    An epitope structure for the C-terminal domain of dystrophin and utrophin.
    Nguyen thi Man
    Biochemistry 37.31 (1998 Aug 4): 11117-27.

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