Catalog Fields
Antigen: Dystrophin (exon72/73)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody cross-reacts with utrophin.
Predicted Species Reactivity:
Gene: DMD
Immunogen: Recombinant dystrophin fragment
Alternate Gene Names: BMD
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 3436-3450
Uniprot ID: P11532
Immunogen Sequence: Exon 72/73
Entrez Gene ID: 1756
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm
Antibody Registry ID: AB_2618131
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANCHO11(9F2) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANCHO11(9F2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Localization of the DMDL gene-encoded dystrophin-related protein using a panel of nineteen monoclonal antibodies: presence at neuromuscular junctions, in the sarcolemma of dystrophic skeletal muscle, in vascular and other smooth muscles, and in proliferating brain cell lines.
Morris GE
The Journal of cell biology 115.6 (1991 Dec): 1695-700.