MANCHO11(9F2)
Catalog Fields
Clone ID/Product Name: MANCHO11(9F2)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: DMD
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2618131
Uniprot ID: P11532
Entrez Gene ID: 1756
Clonality: Monoclonal
Immunogen: Recombinant dystrophin fragment
Clone:
Immunogen Sequence: Exon 72/73
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Dystrophin (exon72/73)
Epitope Location or Sequence: a.a. 3436-3450
Alternate Antigen Name:
Deposit Date: 8/9/2017
Antigen Molecular Weight: 426.7 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: Human
Depositor Notes: This antibody cross-reacts with utrophin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANCHO11(9F2) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANCHO11(9F2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
2 References
Initial Publication
Localization of the DMDL gene-encoded dystrophin-related protein using a panel of nineteen monoclonal antibodies: presence at neuromuscular junctions, in the sarcolemma of dystrophic skeletal muscle, in vascular and other smooth muscles, and in proliferating brain cell lines.
Morris GE
The Journal of cell biology 115.6 (1991 Dec): 1695-700.