MANCHO6(5A6)

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$40.00
SKU: MANCHO6(5A6)
View product citations for antibody MANCHO6(5A6) on CiteAb

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Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: Utrophin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Host Species: mouse
Depositors Notes: This antibody is not validated for fish.
Positive Tested Species Reactivity: Canine, Human, Mouse, Xenopus
Gene: UTRN
Antigen Molecular Weight: 394.4 kDa
Alternate Gene Names: DMDL, DRP, DRP1
Immunogen: Recombinant dystrophin fragment
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P46939 
Epitope Location or Sequence: a.a. 3232-3273
Entrez Gene ID: 7402 
Immunogen Sequence: Exon 75
Recommended Applications: Immunofluorescence, Western Blot
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANCHO6(5A6) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANCHO6(5A6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

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