MANDAG1 (7A11)

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$40.00
SKU: MANDAG1 (7A11)
View product citations for antibody MANDAG1 (7A11) on CiteAb

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Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: Dystroglycan, beta
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human, Xenopus
Depositors Notes:
Antigen Molecular Weight: 43 Kda
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: DAG1
Immunogen: Synthetic peptide
Alternate Gene Names: 156DAG, A3a, AGRNR
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: aa-PPY by phage display
Uniprot ID: Q14118 
Immunogen Sequence: C-terminal 15 a.a.
Entrez Gene ID: 1605 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDAG1 (7A11) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDAG1 (7A11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Expression of the 43 kDa dystrophin-associated glycoprotein in human neuromuscular disease.
    Morris GE
    Neuromuscular disorders : NMD 4.2 (1994 Mar): 101-13.

    IF References
    WB References

    Expression of the 43 kDa dystrophin-associated glycoprotein in human neuromuscular disease.
    Morris GE
    Neuromuscular disorders : NMD 4.2 (1994 Mar): 101-13.

    IHC References

    Expression of the 43 kDa dystrophin-associated glycoprotein in human neuromuscular disease.
    Morris GE
    Neuromuscular disorders : NMD 4.2 (1994 Mar): 101-13.

    All References

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