MANDM8 7E7

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$50.00
SKU: MANDM8 7E7

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: MANDM8 7E7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_10804172 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Myotonic Dystrophy Protein Kinase (DMPK) fragment; Epitope: catalytic 355-360
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/8/2010
Antigen Molecular Weight: 55 kDa on SDS-PAGE (does not recognize 80 kDa DMPK by WB)
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: human
Depositor Notes: Cell binding note: reacts with 55 kDa skeletal muscle protein.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rabbit
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDM8 7E7 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDM8 7E7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

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