MANDYS102(7D2)

(0)No Reviews yet
$45.00 to $425.00
SKU: MANDYS102(7D2)
View product citations for antibody MANDYS102(7D2) on CiteAb

In Stock

Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: dystrophin
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Morris, G.E.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Molecular Weight:
Depositors Notes: This antibody does not work with mouse, xenopus, or chicken. Has weak crosscreativity in Immunofluorescence with utrophin.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen:
Gene: DMD
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: Yes
Uniprot ID: P11532 
Epitope Location or Sequence: ex43, 2047-2105
Entrez Gene ID: 1756 
Immunogen Sequence:
Antibody Registry ID: AB_2618155 
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: RRID:AB_2618155
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS102(7D2) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS102(7D2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • All References
  • Initial Publication
    All References

    Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy.
    Morris GE
    American journal of human genetics 52.6 (1993 Jun): 1057-66.

    Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections.
    Morris GE
    The Biochemical journal 288 ( Pt 2).Pt 2 (1992 Dec 1): 663-8.

    A truncated dystrophin lacking the C-terminal domains is localized at the muscle membrane.
    Morris GE
    American journal of human genetics 50.3 (1992 Mar): 508-14.

    The N-terminal half of dystrophin is protected from proteolysis in situ.
    Morris GE
    Biochemical and biophysical research communications 209.3 (1995 Apr 26): 1062-7.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review