(0) No Reviews yet
SKU: MANDYS103(8E11)
View product citations for antibody MANDYS103(8E11) on CiteAb

In Stock

Available: 19

DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin (exon43)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2a
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Host Species: mouse
Depositors Notes: This antibody does not cross react with mouse, xenopus or chick. This antibody is recommended by the depositors.
Positive Tested Species Reactivity: Canine, Human
Gene: DMD
Antigen Molecular Weight: 426.7 kDa
Alternate Gene Names: BMD
Immunogen: Recombinant dystrophin fragment
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P11532 
Epitope Location or Sequence: a.a. 2047-2105
Entrez Gene ID: 1756 
Immunogen Sequence: Exon 43
Recommended Applications: Immunofluorescence, Western Blot
Additional Characterization: 
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS103(8E11) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS103(8E11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Ratings & Reviews

    No reviews available

    Be the first to Write a Review