MANDYS107(4H8)

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$40.00
SKU: MANDYS107(4H8)
View product citations for antibody MANDYS107(4H8) on CiteAb

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Available: 28

DSHB Data Sheet

Catalog Fields

Antigen: dystrophin
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Morris, G.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK
Positive Tested Species Reactivity: Canine, Human
Depositors Notes: This antibody does not work with mouse, xenopus, or chicken.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: DMD
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: ex40/41, 1874-1978
Uniprot ID: P11532 
Immunogen Sequence:
Entrez Gene ID: 1756 
Additional Characterization: Link 
Antibody Registry ID: AB_2618158 
Additional Information: RRID:AB_2618158
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS107(4H8) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS107(4H8))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

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