MANDYS16(1B12)

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$40.00
SKU: MANDYS16(1B12)
View product citations for antibody MANDYS16(1B12) on CiteAb

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Available: 25

DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin, exons 27/28
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Morris, G.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes:
Antigen Molecular Weight: Predicted: 427 kDa; Apparent: 427 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: DMD
Immunogen: Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein
Alternate Gene Names: BMD, CMD3B, MRX85
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0 or Sp2/0
Epitope Location or Sequence: exons 27/28, aa 1226-1245
Uniprot ID: P11532 
Immunogen Sequence: Partial protein
Entrez Gene ID: 1756 
Additional Characterization: Link 
Antibody Registry ID: AB_2618166 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS16(1B12) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS16(1B12))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    FEBS letters
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    IF References

    FEBS letters
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    Methods in molecular biology (Clifton, N.J.)
    Sewry CA
    Methods in molecular biology (Clifton, N.J.) 709. (2011): 39-61.

    The Journal of cell biology
    Partridge TA
    The Journal of cell biology 148.5 (2000 Mar 6): 985-96.

    WB References

    FEBS letters
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    PloS one
    Duan D
    PloS one 9.2 (2014): e88280.

    Methods in molecular biology (Clifton, N.J.)
    Sewry CA
    Methods in molecular biology (Clifton, N.J.) 709. (2011): 39-61.

    Nucleic acids research
    Morris GE
    Nucleic acids research 19.21 (1991 Nov 11): 5889-94.

    ELISA References

    FEBS letters
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    Epitope Map References

    American journal of human genetics
    Morris GE
    American journal of human genetics 52.6 (1993 Jun): 1057-66.

    All References

    FEBS letters
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    Methods in molecular biology (Clifton, N.J.)
    Sewry CA
    Methods in molecular biology (Clifton, N.J.) 709. (2011): 39-61.

    The Journal of cell biology
    Partridge TA
    The Journal of cell biology 148.5 (2000 Mar 6): 985-96.

    PloS one
    Duan D
    PloS one 9.2 (2014): e88280.

    Nucleic acids research
    Morris GE
    Nucleic acids research 19.21 (1991 Nov 11): 5889-94.

    American journal of human genetics
    Morris GE
    American journal of human genetics 52.6 (1993 Jun): 1057-66.

    The Biochemical journal
    Morris GE
    The Biochemical journal 288 ( Pt 2). (1992 Dec 1): 663-8.

    Biochemical and biophysical research communications
    Morris GE
    Biochemical and biophysical research communications 209.3 (1995 Apr 26): 1062-7.

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