MANDYS19(8F6)

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SKU: MANDYS19(8F6)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: MANDYS19(8F6)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: DMD
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2618169 
Uniprot ID: P11532 
RRID:  
Entrez Gene ID: 1756 
Clonality: Monoclonal
Immunogen: Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: NS0 or Sp2/0
Epitope Mapped: Yes
Antigen Name: Dystrophin, exon 21
Epitope Location or Sequence: exon 21. aa 901-904 (GQGP) of isoform 4 (Uniprot ID P11532-1)
Alternate Antigen Name:
Deposit Date: 7/13/2006
Antigen Molecular Weight: Predicted: 427 kDa; Apparent: 427 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: human
Depositor Notes: This antibody does not work with chicken or torpedo
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Human, Mouse
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS19(8F6) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS19(8F6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    Epitope Map References
    All References

    Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin.
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of duchenne muscular dystrophy by immunostaining and western blot.
    Duan D
    PloS one 9.2 (2014): e88280.

    Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies.
    Sewry CA
    Methods in molecular biology (Clifton, N.J.) 709. (2011): 39-61.

    Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibers by clonal expansion.
    Partridge TA
    The Journal of cell biology 148.5 (2000 Mar 6): 985-96.

    Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin.
    Morris GE
    Nucleic acids research 19.21 (1991 Nov 11): 5889-94.

    Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells.
    Pinset C
    Skeletal muscle 5. (2015): 40.

    Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy.
    Morris GE
    American journal of human genetics 52.6 (1993 Jun): 1057-66.

    Monoclonal antibodies for clinical trials of Duchenne muscular dystrophy therapy.
    Morris GE
    Neuromuscular disorders : NMD 24.3 (2014 Mar): 195-200.

    Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections.
    Morris GE
    The Biochemical journal 288 ( Pt 2).Pt 2 (1992 Dec 1): 663-8.

    The N-terminal half of dystrophin is protected from proteolysis in situ.
    Morris GE
    Biochemical and biophysical research communications 209.3 (1995 Apr 26): 1062-7.

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