MANDYS8(8H11)

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$40.00
SKU: MANDYS8(8H11)
View product citations for antibody MANDYS8(8H11) on CiteAb

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Available: 34

DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin, exons 31/32
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, UK
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Notes: Doesn't work on Xenopus. Fixation for immunostaining: acetone/methanol.
Antigen Molecular Weight: Predicted: 427 kDa; Apparent: 427 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: DMD
Immunogen: Recombinant LacZ/dystrophin (aa 816-1749, rod domain of isoform 4 (Uniprot ID P11532-1)) fusion protein
Alternate Gene Names: BMD, CMD3B, MRX85
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: exons 31/32, aa 1431-1505
Uniprot ID: P11532 
Immunogen Sequence: Partial protein
Entrez Gene ID: 1756 
Additional Characterization: Link 
Antibody Registry ID: AB_2618170 
Additional Information: The collection of anti-DMD mAbs deposited by Morris, G.E., are directed against different DMD exons.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANDYS8(8H11) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANDYS8(8H11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    Epitope Map References
    All References

    Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin.
    Davies KE
    FEBS letters 262.2 (1990 Mar 26): 237-40.

    Phase I study of dystrophin plasmid-based gene therapy in Duchenne/Becker muscular dystrophy.
    Fardeau M
    Human gene therapy 15.11 (2004 Nov): 1065-76.

    Strand bias in oligonucleotide-mediated dystrophin gene editing.
    Rando TA
    Human molecular genetics 14.2 (2005 Jan 15): 221-33.

    Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of duchenne muscular dystrophy by immunostaining and western blot.
    Duan D
    PloS one 9.2 (2014): e88280.

    Monitoring duchenne muscular dystrophy gene therapy with epitope-specific monoclonal antibodies.
    Sewry CA
    Methods in molecular biology (Clifton, N.J.) 709. (2011): 39-61.

    Myoblast transfer in the treatment of Duchenne's muscular dystrophy.
    Rice R
    The New England journal of medicine 333.13 (1995 Sep 28): 832-8.

    Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibers by clonal expansion.
    Partridge TA
    The Journal of cell biology 148.5 (2000 Mar 6): 985-96.

    Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin.
    Morris GE
    Nucleic acids research 19.21 (1991 Nov 11): 5889-94.

    Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy.
    Morris GE
    American journal of human genetics 52.6 (1993 Jun): 1057-66.

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