MANEM2(9B2)

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$50.00 to $600.00
SKU: MANEM2(9B2)

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Product Name/ID: MANEM2(9B2)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: EMD
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2753288 
Uniprot ID: P50402 
RRID:  
Entrez Gene ID: 2010 
Clonality: Monoclonal
Immunogen: Recombinant dystrophin fragment
Clone:
Immunogen Sequence: N-terminus a.a. 1-188
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Emerin
Epitope Location or Sequence: a.a. 89-96
Alternate Antigen Name:
Deposit Date: 8/9/2017
Antigen Molecular Weight: 28.9 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: Human
Depositor Notes: Loss or mutations in this antigen is associated with Emery-Dreifuss Muscular Dystrophy. This antibody does not recognize mouse emerin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rabbit
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEM2(9B2) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEM2(9B2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    The Emery-Dreifuss muscular dystrophy protein, emerin, is a nuclear membrane protein.
    Morris GE
    Human molecular genetics 5.6 (1996 Jun): 801-8.

    IF References
    WB References

    The Emery-Dreifuss muscular dystrophy protein, emerin, is a nuclear membrane protein.
    Morris GE
    Human molecular genetics 5.6 (1996 Jun): 801-8.

    Epitope Map References
    All References

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