MANEM7(7A9)

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$40.00
SKU: MANEM7(7A9)
View product citations for antibody MANEM7(7A9) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Emerin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human
Depositors Notes: Loss or mutations in this antigen is associated with Emery-Dreifuss Muscular Dystrophy. This antibody does not recognize mouse or rabbit emerin.
Antigen Molecular Weight: 28.9 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: EMD
Immunogen: Recombinant dystrophin fragment
Alternate Gene Names: LEMD5, STA
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 7-17
Uniprot ID: P50402 
Immunogen Sequence: N-terminus a.a. 1-188
Entrez Gene ID: 2010 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEM7(7A9) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEM7(7A9))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    The Emery-Dreifuss muscular dystrophy protein, emerin, is a nuclear membrane protein.
    Morris GE
    Human molecular genetics 5.6 (1996 Jun): 801-8.

    IF References
    WB References

    The Emery-Dreifuss muscular dystrophy protein, emerin, is a nuclear membrane protein.
    Morris GE
    Human molecular genetics 5.6 (1996 Jun): 801-8.

    Epitope Map References
    All References

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