MANEX44B(10G5)

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$45.00 to $425.00
SKU: MANEX44B(10G5)
View product citations for antibody MANEX44B(10G5) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin (exon44)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: Morris, G.E.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Molecular Weight: 426.7 kDa
Depositors Notes: This antibody is not validated for xenopus, and cross-reacts with utrophin. This antibody is recommended by the depositors.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant dystrophin fragment
Gene: DMD
Alternate Antibody Name:
Alternate Gene Names: BMD
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: P11532 
Epitope Location or Sequence:
Entrez Gene ID: 1756 
Immunogen Sequence: Exon 44
Antibody Registry ID: AB_2732017 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: RRID: AB_2732017
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEX44B(10G5) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEX44B(10G5))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
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