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View product citations for antibody MANEX44B(10G5) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin (exon44)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Host Species: mouse
Depositors Notes: This antibody is not validated for xenopus, and cross-reacts with utrophin. This antibody is recommended by the depositors.
Positive Tested Species Reactivity: Human
Gene: DMD
Antigen Molecular Weight: 426.7 kDa
Alternate Gene Names: BMD
Immunogen: Recombinant dystrophin fragment
Clonality: Monoclonal
Myeloma Strain: SP2/0
Immunogen Sequence: Exon 44
Uniprot ID: P11532 
Additional Characterization: 
Entrez Gene ID: 1756 
Additional Information: RRID: AB_2732017
Antibody Registry ID: AB_2732017 
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEX44B(10G5) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEX44B(10G5))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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