MANEX7374D(4G10)

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$40.00
SKU: MANEX7374D(4G10)
View product citations for antibody MANEX7374D(4G10) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin (exon73/74)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: This antibody is not validated for xenopus.
Antigen Molecular Weight: 426.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: DMD
Immunogen: Recombinant dystrophin fragment
Alternate Gene Names: BMD
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 3445-3517
Uniprot ID: P11532 
Immunogen Sequence: Exon 73/74
Entrez Gene ID: 1756 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEX7374D(4G10) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEX7374D(4G10))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    IF References

    [Cytomorphological and cytochemical characteristics of primary pulmonary carcinoma (author's transl)].
    Mermolja M
    Plucne bolesti i tuberkuloza 30.3 (1978 Jul-Sep): 194-8.

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    WB References

    [Cytomorphological and cytochemical characteristics of primary pulmonary carcinoma (author's transl)].
    Mermolja M
    Plucne bolesti i tuberkuloza 30.3 (1978 Jul-Sep): 194-8.

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    All References

    [Cytomorphological and cytochemical characteristics of primary pulmonary carcinoma (author's transl)].
    Mermolja M
    Plucne bolesti i tuberkuloza 30.3 (1978 Jul-Sep): 194-8.

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

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