MANEX7374M(7E10)

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$40.00
SKU: MANEX7374M(7E10)
View product citations for antibody MANEX7374M(7E10) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Dystrophin (exon73/74)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Host Species: mouse
Gene: DMD
Positive Tested Species Reactivity: Human, Mouse, Xenopus
Alternate Gene Names: BMD
Antigen Molecular Weight: 426.7 kDa
Clonality: Monoclonal
Immunogen: Recombinant dystrophin fragment
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 3445-3517
Uniprot ID: P11532 
Immunogen Sequence: Exon 73/74
Entrez Gene ID: 1756 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEX7374M(7E10) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEX7374M(7E10))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    IF References

    [Cytomorphological and cytochemical characteristics of primary pulmonary carcinoma (author's transl)].
    Mermolja M
    Plucne bolesti i tuberkuloza 30.3 (1978 Jul-Sep): 194-8.

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    WB References

    [Cytomorphological and cytochemical characteristics of primary pulmonary carcinoma (author's transl)].
    Mermolja M
    Plucne bolesti i tuberkuloza 30.3 (1978 Jul-Sep): 194-8.

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    All References

    [Cytomorphological and cytochemical characteristics of primary pulmonary carcinoma (author's transl)].
    Mermolja M
    Plucne bolesti i tuberkuloza 30.3 (1978 Jul-Sep): 194-8.

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

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