MANEX7374R(8A12)

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$45.00 to $425.00
SKU: MANEX7374R(8A12)
View product citations for antibody MANEX7374R(8A12) on CiteAb

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Available: 20

DSHB Data Sheet

Catalog Fields

Antigen: dystrophin
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: Morris, G.E.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Molecular Weight:
Depositors Notes: This antibody does not work with Xenopus
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen:
Gene: DMD
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: Yes
Uniprot ID: P11532 
Epitope Location or Sequence: ex73/74, 3445-3517
Entrez Gene ID: 1756 
Immunogen Sequence:
Antibody Registry ID: AB_2618187 
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: RRID:AB_2618187
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEX7374R(8A12) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEX7374R(8A12))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • All References
  • Initial Publication

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    All References

    Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
    Nguyen TM
    Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.

    The N-terminal half of dystrophin is protected from proteolysis in situ.
    Morris GE
    Biochemical and biophysical research communications 209.3 (1995 Apr 26): 1062-7.

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