Catalog Fields
Antigen: Dystrophin (exon73/74)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: This antibody is not validated for xenopus.
Predicted Species Reactivity:
Gene: DMD
Immunogen: Recombinant dystrophin fragment
Alternate Gene Names: BMD
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 3445-3517
Uniprot ID: P11532
Immunogen Sequence: Exon 73/74
Entrez Gene ID: 1756
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm
Antibody Registry ID: AB_2753279
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEX7374S(10A6) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEX7374S(10A6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
Nguyen TM
Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.