MANEX7374S(10A6)
Catalog Fields
Antigen: Dystrophin (exon73/74)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Host Species: mouse
Depositors Notes: This antibody is not validated for xenopus.
Positive Tested Species Reactivity: Human, Mouse
Gene: DMD
Antigen Molecular Weight: 426.7 kDa
Alternate Gene Names: BMD
Immunogen: Recombinant dystrophin fragment
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P11532
Epitope Location or Sequence: a.a. 3445-3517
Entrez Gene ID: 1756
Immunogen Sequence: Exon 73/74
Recommended Applications: Immunofluorescence, Western Blot
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANEX7374S(10A6) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANEX7374S(10A6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Apo-dystrophins (Dp140 and Dp71) and dystrophin splicing isoforms in developing brain.
Nguyen TM
Biochemical and biophysical research communications 215.1 (1995 Oct 4): 361-7.