MANFU10-5H10

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$40.00
SKU: MANFU10-5H10
View product citations for antibody MANFU10-5H10 on CiteAb

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Available: 180

DSHB Data Sheet

Catalog Fields

Antigen: Fukutin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease
Host Species: mouse
Depositors Notes: This antibody recognizes a glycosyltransferase in the cis-Golgi compartment. Fukutin is thought to be responsible for the glycosylation of alpha-dystroglycan in skeletal muscle.
Positive Tested Species Reactivity: Human
Gene: FKTN
Antigen Molecular Weight: 53.7 kDa
Alternate Gene Names: FCMD, LGMD2M
Immunogen: Recombinant Protein
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Uniprot ID: O75072 
Epitope Location or Sequence: a.a. 180-187 (SGNYL - HG)
Entrez Gene ID: 2218 
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_2618191 
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: Epitope mapped by phage display. RRID:AB_2618191
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANFU10-5H10 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANFU10-5H10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    WB References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    Epitope Map References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    All References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

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